Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry
文献类型：期刊论文
| 作者 | Zhao, Jieyu; Hu, Chunxiu; Zeng, Jun; Zhao, Yanni; Zhang, Junjie; Chang, Yuwei; Li, Lili; Zhao, Chunxia; Lu, Xin; Xu, Guowang |
| 刊名 | metabolomics
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 10期号:5页码:805-815 |
| 关键词 | Capillary electrophoresis Mass spectrometry Plant metabolomics Method validation Tobacco leaf |
| 通讯作者 | 许国旺 |
| 英文摘要 | many metabolites in plant are highly polar and ionic. their analysis using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry can be problematic. therefore a capillary electrophoresis-mass spectrometry (ce-ms) method with charge-driven separation characteristic was developed to investigate polar metabolites in tobacco. to obtain as many features as possible, extraction of polar metabolites was optimized by the design of experiments and evaluated by univariate statistics. method validation was carried out to evaluate the analytical characteristics including calibration curve, precision, sample stability and extraction reproducibility. the developed method was successfully applied in studying 30 tobacco leaves obtained from yunnan and guizhou provinces in china. a total of 154 polar metabolites were identified based on available database. multivariate pattern recognition clearly revealed the metabolic differences between the two geographic areas and 43 significantly different metabolites were defined by the non-parametric hypothesis test (mann-whitney u test) and false discovery rate. some key metabolites involved in photosynthesis such as ribulose 1,5-disphosphate, fructose 1,6-diphosphate, glycine, betaine, gaba and serine were found to be susceptible to environmental conditions. this study shows that the metabolic profiling based on ce-ms can clearly discriminate tobacco leaves of different geographical origins and understand the relationship between plant metabolites and their geographical origins. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | endocrinology & metabolism |
| 研究领域[WOS] | endocrinology & metabolism |
| 关键词[WOS] | solid-phase microextraction ; false discovery rate ; liquid-chromatography ; gas-chromatography ; ce-ms ; metabolomics ; extraction ; plant ; leaves ; optimization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341422600004 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144497]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jieyu,Hu, Chunxiu,Zeng, Jun,et al. Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry[J]. metabolomics,2014,10(5):805-815. |
| APA | Zhao, Jieyu.,Hu, Chunxiu.,Zeng, Jun.,Zhao, Yanni.,Zhang, Junjie.,...&Xu, Guowang.(2014).Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry.metabolomics,10(5),805-815. |
| MLA | Zhao, Jieyu,et al."Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry".metabolomics 10.5(2014):805-815. |
入库方式： OAI收割
来源：大连化学物理研究所
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