Improved islet purity by the hypertonic-hypotonic method
文献类型：期刊论文
| 作者 | Li, Na1,2,3; Zhang, Ying1; Liu, Yang1; Xiu, Zhilong2; Tan, Mingqian1; Li, Shen1,2; Guo, Xin1; Wang, Yu1,3; Chen, Li1,3; Song, Yizhe1,3 |
| 刊名 | international journal of artificial organs
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 37期号:6页码:477-485 |
| 关键词 | Islets isolation Purification Hypertonic-hypotonic method Plackett-Burman method Density gradient separation |
| 通讯作者 | 张英 |
| 英文摘要 | introduction: islet purification is usually performed using the density gradient separation method, but the purity of islets is low because exocrine cells and the embedded islets are hard to remove by using only the density gradient method. the aim of this study was to establish a new islet purification process comprising a hypertonic-hypotonic treatment step followed by a density gradient centrifugation step to improve the purity of islets. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; technology ; life sciences & biomedicine |
| 类目[WOS] | engineering, biomedical ; transplantation |
| 研究领域[WOS] | engineering ; transplantation |
| 关键词[WOS] | cell transplantation ; pancreatic-islets ; purification ; optimization ; osmolality ; langerhans ; iodixanol ; survival ; density ; ficoll |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343850600006 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144518]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Zhang, Ying,Liu, Yang,et al. Improved islet purity by the hypertonic-hypotonic method[J]. international journal of artificial organs,2014,37(6):477-485. |
| APA | Li, Na.,Zhang, Ying.,Liu, Yang.,Xiu, Zhilong.,Tan, Mingqian.,...&Ma, Xiaojun.(2014).Improved islet purity by the hypertonic-hypotonic method.international journal of artificial organs,37(6),477-485. |
| MLA | Li, Na,et al."Improved islet purity by the hypertonic-hypotonic method".international journal of artificial organs 37.6(2014):477-485. |
入库方式： OAI收割
来源：大连化学物理研究所
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