In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis
文献类型：期刊论文
| 作者 | Huang, Junfeng1,2; Qin, Hongqiang1; Dong, Jing1; Song, Chunxia1; Bian, Yangyang1,2; Dong, Mingming1,2; Cheng, Kai1,2; Wang, Fangjun1; Sun, Deguang3; Wang, Liming3 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 13期号:9页码:3896-3904 |
| 关键词 | in situ sample processing approach phosphoprotein enrichment on-beads digestion dimethyl labeling solid phase labeling phosphoproteome quantification human liver hepatocellular carcinoma |
| 通讯作者 | 叶明亮 ; 邹汉法 |
| 英文摘要 | current sample preparation protocols for quantitative phosphoproteome analysis are tedious and time-consuming. here, a facile in situ sample processing approach (ispa) is developed by using macroporous ti(iv)-imac microspheres as the preparation "beds", where all sample preparation procedures including the enrichment of phosphoproteins, tryptic digestion of proteins, enrichment, and isotope labeling of phosphopeptides are performed in situ sequentially. as a result of the in situ processing design and the seamless procedures, extra steps for desalting and buffer exchanging, which are always required in conventional approaches, are avoided, and the sample loss and contamination could be greatly reduced. thus, better sensitivity and accuracy for the quantitative phosphoproteome analysis were obtained. this strategy was further applied to differential phosphoproteome analysis of human liver tissues with or without hepatocellular carcinoma (hcc). in total, 8548 phosphorylation sites were confidently quantified from three replicate analyses of 0.5 mg of human liver protein extracts. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | ion affinity-chromatography ; phosphorylated proteins ; mass-spectrometry ; selective enrichment ; phosphopeptides ; quantification ; liver ; identification ; adsorbent ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341345000004 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144524]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Junfeng,Qin, Hongqiang,Dong, Jing,et al. In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis[J]. journal of proteome research,2014,13(9):3896-3904. |
| APA | Huang, Junfeng.,Qin, Hongqiang.,Dong, Jing.,Song, Chunxia.,Bian, Yangyang.,...&Zou, Hanfa.(2014).In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis.journal of proteome research,13(9),3896-3904. |
| MLA | Huang, Junfeng,et al."In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis".journal of proteome research 13.9(2014):3896-3904. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。