Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Li, Cunman1; Zhao, Yanyan2; Guo, Zhimou3; Zhang, Xiuli3; Xue, Xingya3; Liang, Xinmiao3 |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 99期号:1页码:35-44 |
| 关键词 | Micro-component UPLC-DAD/Q-TOF MS 2D-RPLC/RPLC Click OEG stationary phase Hedyotis diffusa Willd |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | an effective method aiming at enrichment and analysis of micro-components in traditional chinese medicine (tcm) was developed. one fraction (fraction e) from the extract of hedyotis diffusa willd. was selected as test sample, which was isolated by using the xad-4 macroporous resin. to study the micro-components, a two-dimensional reverse-phase liquid chromatography (2d-rplc/rplc) method was developed, comprising click oeg and c18 stationary phases as the first and second dimensions, respectively. of the eight sub-fractions isolated from the first dimension, three sub-fractions (fractions ii-iv) containing micro-components were further separated with the second dimension. the 2d-rplc/rplc system was proved to possess high orthogonality. furthermore, the micro-components were characterized by using ultra-performance liquid chromatography-diode array detector/quadrupole time-of-flight mass spectrometry (uplc-dad/q-tof ms) with electrospray ionization (esi) source. with the optimized separation and characterization method, a large number (>400) of micro-components were enriched and detected from the extracts of h. diffusa willd., the majority of which has not been isolated from the herb before. among these isolated micro-components, 38 compounds involving 24 phenylpropanoids, 7 flavonoids and 7 iridoid glucosides (igs), were identified or tentatively identified from the h. diffusa extracts on the basis of spectral data of the authentic standards and the fragmentation characteristics information available in literatures. the proposed method made it possible to effectively screen and analyze the micro-components in tcms or other complex natural medicines. (c) 2014 elsevier b.v. all rights reserved. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | traditional chinese medicine ; iridoid glucosides ; electrospray-ionization ; oligo(ethylene glycol) ; stationary-phase ; click ; constituents ; glycosides ; corymbosa ; column |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341169100006 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144526]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Hebei Univ Sci & Technol, Hebei Res Ctr Anal & Testing, Shijiazhuang 050018, Peoples R China 2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Cunman,Zhao, Yanyan,Guo, Zhimou,et al. Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. journal of pharmaceutical and biomedical analysis,2014,99(1):35-44. |
| APA | Li, Cunman,Zhao, Yanyan,Guo, Zhimou,Zhang, Xiuli,Xue, Xingya,&Liang, Xinmiao.(2014).Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.journal of pharmaceutical and biomedical analysis,99(1),35-44. |
| MLA | Li, Cunman,et al."Effective 2D-RPLC/RPLC enrichment and separation of micro-components from Hedyotis diffusa Willd. and characterization by using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry".journal of pharmaceutical and biomedical analysis 99.1(2014):35-44. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。