A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot
文献类型：期刊论文
| 作者 | Hou, Jie1,2; Shi, Qian2; Cao, Meirong2; Pan, Pengwei2; Ge, Guangbo3; Fan, Xuran3; Bai, Gang2; Xin, Yi1 |
| 刊名 | biochemistry-moscow
 |
| 出版日期 | 2014 |
| 卷号 | 79期号:1页码:25-30 |
| 关键词 | fluorescent visualization glycyrrhetic acid quantum dot click reaction |
| 英文摘要 | glycyrrhetic acid (ga), a pentacyclic triterpenoid derivative obtained from hydrolysis of glycyrrhizic acid, was found to have synergistic anti-asthmatic effects with the beta(2)-adrenergic receptor (beta(2)ar) agonist via the beta(2)ar-mediated pathway. this study visualized the location of ga on a human cell expressing beta(2)ar via chemical biological approaches. a cdte/zns quantum dot modified with an alkynyl group (qd-al) was first synthesized, and an azide-terminal ga (atga) was also prepared. the qd-al was used for fluorescence visualization of the distribution of ga on human embryonic kidney 293 cells expressing fusion beta(2)ar (hek293-beta(2)ar) through the "click reaction" between qd-al and atga. the average size of the qd-al nanoparticle was about 10 nm, and its fluorescent emission wavelength was 620 nm. the location of ga on the hek293-beta(2)ar cell membrane can be visualized by the click reaction (between qd-al and atga). the ability of qd-al targeting to atga on the cell membrane of a hek293-beta(2)ar cell was further investigated using both confocal laser-scanning microscopy and a cellular uptake-inhibition assay. the results reveal that qd-al can recognize atga on the cell membrane through the click reaction, which provides a new approach for visualizing the location of ga on the cell in an indirect way, and it can be applied to explore the synergistic anti-asthmatic mechanism of ga with beta(2)ar agonist through the beta(2)ar mediated pathway. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | receptor agonist ; nanocrystals ; phase |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330962200004 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145412]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China 2.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jie,Shi, Qian,Cao, Meirong,et al. A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot[J]. biochemistry-moscow,2014,79(1):25-30. |
| APA | Hou, Jie.,Shi, Qian.,Cao, Meirong.,Pan, Pengwei.,Ge, Guangbo.,...&Xin, Yi.(2014).A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot.biochemistry-moscow,79(1),25-30. |
| MLA | Hou, Jie,et al."A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot".biochemistry-moscow 79.1(2014):25-30. |
入库方式： OAI收割
来源：大连化学物理研究所
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