New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC
文献类型：期刊论文
| 作者 | Wang, Lina1; Quan, Chunshan2,3; Xiong, Wen2,3; Qu, Xiaojing2,3; Fan, Shengdi2,3; Hu, Wenzhong2,3 |
| 刊名 | biochimica et biophysica acta-biomembranes
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 1838期号:3页码:988-993 |
| 关键词 | AgrC Topology GFP SCAM Staphylococcus aureus |
| 英文摘要 | staphylococcus aureus accessory gene regulator (agr) locus controls the expression of virulence factors through a classical two-component signal transduction system that consists of a receptor histidine protein kinase agrc and a cytoplasmic response regulator agra. an autoinducing peptide (aip) encoded by agr locus activates agrc, which transduces extracellular signals into the cytoplasm. despite extensive investigations to identify agrc-aip interaction sites, precise signal recognition mechanisms remain unknown. this study aims to clarify the membrane topology of agrc by applying the green fluorescent protein (gfp) fusion technique and the substituted cysteine accessibility method (scam). however, our findings were inconsistent with profile obtained previously by alkaline phosphatase. we report the topology of agrc shows seven transmembrane segments, a periplasmic n-terminus, and a cytoplasmic c-terminus. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biophysics |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics |
| 关键词[WOS] | cysteine accessibility method ; membrane-protein topogenesis ; virulence genes ; regulatory rna ; united-states ; rf cloning ; receptor ; prediction ; recognition ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331661800029 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145426]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China 3.State Ethn Affairs Commiss, Minist Educ Econ & Technol Dev Zone, Dalian 116600, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lina,Quan, Chunshan,Xiong, Wen,et al. New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC[J]. biochimica et biophysica acta-biomembranes,2014,1838(3):988-993. |
| APA | Wang, Lina,Quan, Chunshan,Xiong, Wen,Qu, Xiaojing,Fan, Shengdi,&Hu, Wenzhong.(2014).New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC.biochimica et biophysica acta-biomembranes,1838(3),988-993. |
| MLA | Wang, Lina,et al."New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC".biochimica et biophysica acta-biomembranes 1838.3(2014):988-993. |
入库方式： OAI收割
来源：大连化学物理研究所
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