Highly Efficient Preparation of Bisphenol A Glucuronide
文献类型：期刊论文
| 作者 | Yang Gang1; Zhu Liangliang2; Lu Xia2; Wu Dachang1; Xia Yangliu2; Ma Xiaochi1; Xin Yi1; Hou Jie1 |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2014-02-10 |
| 卷号 | 35期号:2页码:314-318 |
| 关键词 | Bisphenol A Rat liver microsome Bisphenol A-beta-O-glucuronide Solid-phase extraction |
| 英文摘要 | bisphenol a(bpa), an industrial chemical, has been present in many hard plastic bottles, metal-based food and beverage cans. many governments are trying to reduce the human exposure to bpa because it is really a readily accessible toxin for human. bpa can be extensively metabolized to bisphenol a-beta-o-glucuronide(bpa-g) by udp-glucuronosyltransferases(ugts), thus the biological activities of bpa-g should be studied intensively. however, the studies on bpa-g are very limited due to the difficulty for preparation of bpa-g. in this work, the bpa-g was prepared using bpa as the starting material and rat liver microsomes (rlm) as the enzyme source. a series of orthogonal experiments was designed and performed to determine. the optimal reaction conditions for bpa glucuronidation in rlm. after incubation under the optimal reaction conditions, the conversion of bpa was higher than 98%. a unique solid-phase extraction column (spe) packed with c-18 wax was used to enrich and purify the product bpa-g with high yield. finally, the product was identified by both esi-ms and nmr techniques. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; liver ; diethylstilbestrol ; intestine ; isoforms ; exposure ; adult |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332918400018 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145457]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Dept Biotechnol, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Gang,Zhu Liangliang,Lu Xia,et al. Highly Efficient Preparation of Bisphenol A Glucuronide[J]. chemical journal of chinese universities-chinese,2014,35(2):314-318. |
| APA | Yang Gang.,Zhu Liangliang.,Lu Xia.,Wu Dachang.,Xia Yangliu.,...&Hou Jie.(2014).Highly Efficient Preparation of Bisphenol A Glucuronide.chemical journal of chinese universities-chinese,35(2),314-318. |
| MLA | Yang Gang,et al."Highly Efficient Preparation of Bisphenol A Glucuronide".chemical journal of chinese universities-chinese 35.2(2014):314-318. |
入库方式： OAI收割
来源：大连化学物理研究所
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