Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma
文献类型：期刊论文
| 作者 | Wang, Jixia1; Kong, Song2; Yan, Jingyu1; Jin, Gaowa1; Guo, Zhimou1; Shen, Aijin1; Xu, Junyan1; Zhang, Xiuli1; Zou, Lijuan2; Liang, Xinmiao1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 960页码:214-221 |
| 关键词 | HILIC-SPE PPT RPLC-MS/MS Triptorelin Plasma |
| 英文摘要 | peptide drugs play a critical role in therapeutic treatment. however, as the complexity of plasma, determination of peptide drugs using liquid chromatography-tandem mass spectrometry (lc-ms/ms) is a daunting task. to solve this problem, hydrophilic interaction liquid chromatography-solid phase extraction (hilic-spe) directly combined with protein precipitation (ppt) was developed for the selective extraction of triptorelin from plasma. the extracts were analyzed by reversed-phase liquid chromatography (rplc). proteins, phospholipids and highly polar interferences could be removed from plasma by the efficient combination of ppt, hilic-spe and rplc-ms/ms. this method was evaluated by matrix effect, recovery and process efficiency at different concentration levels (50, 500 and 5000 ng/ml) of triptorelin. furthermore, the performance of hilic-spe was compared with that of reversed-phase c18 spe and hydrophilic lipophilic balance (oasis hlb) spe. among them, hilic-spe provided the minimum matrix effect (ranging from 96.02% to 103.41%), the maximum recovery (ranging from 80.68% to 90.54%) and the satisfactory process efficiency (ranging from 82.83% to 92.95%). the validated method was successfully applied to determine triptorelin in rat plasma. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; sample preparation ; reversed-phase ; electrospray-ionization ; biological fluid ; ion suppression ; matrix ; quantification ; peptides ; phospholipids |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336821600030 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145478]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dept Radiat Oncol, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jixia,Kong, Song,Yan, Jingyu,et al. Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2014,960:214-221. |
| APA | Wang, Jixia.,Kong, Song.,Yan, Jingyu.,Jin, Gaowa.,Guo, Zhimou.,...&Liang, Xinmiao.(2014).Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma.journal of chromatography b-analytical technologies in the biomedical and life sciences,960,214-221. |
| MLA | Wang, Jixia,et al."Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma".journal of chromatography b-analytical technologies in the biomedical and life sciences 960(2014):214-221. |
入库方式： OAI收割
来源：大连化学物理研究所
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