Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells
文献类型：期刊论文
| 作者 | Song, Huiyi1,3; Yu, Weiting1; Liu, Xiudong2; Ma, Xiaojun1 |
| 刊名 | carbohydrate polymers
 |
| 出版日期 | 2014-08-08 |
| 卷号 | 108页码:10-16 |
| 关键词 | Microencapsulated cell culture Probiotic viability Resistance to stress environments |
| 英文摘要 | in this study, probiotics (saccharomyces cerevisiae y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. two different encapsulation patterns were established as directly entrapped high density cells (dehdc) and entrapped low density cells with culture (eldcwc). the performance of microencapsulated cells, with free cells (fc) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. after being freeze-dried without cryoprotectant, the survival rate of eldcwc (14.33%) was significantly higher than 10.00% of dehdc, and 0.05% of fc. the lower temperature (-20 degrees c) and eldcwc pattern were beneficial for keeping viable cells at 7.00 log cfu g-(1) after 6 months. furthermore, the eldcwc microcapsule maintained viable cells of 6.29 log cfu g(-1) after incubation in sgf and sif. these studies demonstrated that the pattern of entrapped low density cells with culture was an effective and superior technique of resisting harmful stress environments. (c) 2014 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, applied ; chemistry, organic ; polymer science |
| 研究领域[WOS] | chemistry ; polymer science |
| 关键词[WOS] | simulated gastrointestinal conditions ; lactic-acid bacteria ; lactobacillus-acidophilus ; survival ; storage ; encapsulation ; stability ; cryoprotectants ; bifidobacterium ; microspheres |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336193600002 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145487]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, PR, Peoples R China 2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Huiyi,Yu, Weiting,Liu, Xiudong,et al. Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells[J]. carbohydrate polymers,2014,108:10-16. |
| APA | Song, Huiyi,Yu, Weiting,Liu, Xiudong,&Ma, Xiaojun.(2014).Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.carbohydrate polymers,108,10-16. |
| MLA | Song, Huiyi,et al."Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells".carbohydrate polymers 108(2014):10-16. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。