Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis
文献类型：期刊论文
| 作者 | Pan, Yanbo1,2; Ye, Mingliang1; Zheng, Hao1,2; Cheng, Kai1,2; Sun, Zhen1,2; Liu, Fangjie1,2; Liu, Jing1,2; Wang, Keyun1,2; Qin, Hongqiang1; Zou, Hanfa1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014-01-21 |
| 卷号 | 86期号:2页码:1170-1177 |
| 英文摘要 | an enzymatic approach to label peptide n-termini with isotope-coded affinity tags is presented. this method exploits the high activity of trypsin for peptide synthesis in organic solvents. a cosubstrate containing a stable isotope-coded arg residue and a biotin tag was synthesized. when the cosubstrate was incubated with tryptic peptides and trypsin in ethanol solution, the stable isotope-coded affinity tag was specifically coupled onto the n-termini of peptides via the formation of new peptide bonds. the labeled peptides were specifically enriched by avidin affinity chromatography and then were submitted to liquid chromatography tandem mass spectrometry (lc/ms/ms) for quantification. this enrichment step effectively reduced the interference by unlabeled peptides. the excellent performance of this approach was demonstrated by labeling standard peptides as well as a mouse liver digest. in addition to one amino acid residue, a few dipeptide tags were also introduced to the n-termini of peptides successfully by this enzymatic approach. it was found that the identifications for samples labeled with these tags were highly complementary. coupling a short sequence tag onto peptides could be an effective approach to improve the coverage for proteome analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; quantitative proteomics ; sulfonated peptides ; isobaric tags ; quantification ; guanidination ; dissociation ; fragments |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330204500029 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145495]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Yanbo,Ye, Mingliang,Zheng, Hao,et al. Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis[J]. analytical chemistry,2014,86(2):1170-1177. |
| APA | Pan, Yanbo.,Ye, Mingliang.,Zheng, Hao.,Cheng, Kai.,Sun, Zhen.,...&Zou, Hanfa.(2014).Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis.analytical chemistry,86(2),1170-1177. |
| MLA | Pan, Yanbo,et al."Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis".analytical chemistry 86.2(2014):1170-1177. |
入库方式： OAI收割
来源：大连化学物理研究所
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