Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium
文献类型：期刊论文
| 作者 | Wang, Xiaohui1,2,3,5; Zhao, Yong1; Tan, Haidong1; Chi, Naiyu2; Zhang, Qingfang2; Du, Yuguang4; Yin, Heng1 |
| 刊名 | international journal of biological macromolecules
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 70页码:455-462 |
| 关键词 | Chitinase Purification Characterisation |
| 英文摘要 | in this study, we isolated a new psychrophilic bacterium, pseudoalteromonas sp. dl-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees c. one endo-type chitinase gene, chia, was cloned from the genomic dna of this bacterium and heterologously expressed in escherichia coli bl21 (de3). chia showed very high catalytic activity, even at 4 degrees c, and exhibited maximal activity on a chitinous substrate at ph 8.0 and 20 degrees c. kinetic studies indicated that chia has a greater catalytic efficiency on 4-methylumbelliferyl-beta-d-n,n',n"-triacetylchitotriose[4-mu(glcnac)(3)] than on 4-methylumbelliferyl-beta-d-n,n'-diacetylchitobioside[4-mu(glcnac)(2)]. electrospray ionisation mass spectrometry (esi-ms) analysis showed that the hydrolysis products of powdered chitin after chia digestion consisted of a series of chitin oligomers with different degrees of polymerisation. the chia mode of action was also examined using (glcnac)(2-6) as a substrate, and the results suggested that chia is a non-processive endo-type chitinase. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | sp strain o-7 ; serratia-marcescens ; recalcitrant polysaccharides ; family-18 chitinase ; molecular analysis ; moritella-marina ; binding domain ; gene ; processivity ; degradation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342478800065 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145567]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohui,Zhao, Yong,Tan, Haidong,et al. Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium[J]. international journal of biological macromolecules,2014,70:455-462. |
| APA | Wang, Xiaohui.,Zhao, Yong.,Tan, Haidong.,Chi, Naiyu.,Zhang, Qingfang.,...&Yin, Heng.(2014).Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium.international journal of biological macromolecules,70,455-462. |
| MLA | Wang, Xiaohui,et al."Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium".international journal of biological macromolecules 70(2014):455-462. |
入库方式： OAI收割
来源：大连化学物理研究所
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