Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions
文献类型：期刊论文
| 作者 | Li, Lili; Zhao, Chunxia; Chang, Yuwei; Lu, Xin; Zhang, Junjie; Zhao, Yanni; Zhao, Jieyu; Xu, Guowang |
| 刊名 | journal of separation science
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 37期号:9-10页码:1067-1074 |
| 关键词 | Chemical fingerprint Cured tobacco Plant metabolomics Regional variation MS MS |
| 英文摘要 | cured tobacco is an important plant material. component studies are a big challenge for its significantly diverse chemical properties and vastly different concentrations. in this work, liquid chromatography with quadrupole time-of-flight mass spectrometry was used to perform a metabolomics study of cured tobacco owing to its efficient separation and detection of semipolar metabolites. a solvent of methanol/water (8:2, v/v) and 30 min of ultrasound time were found to be optimal to perform extraction. 95, 92, and 93% of metabolite features had within 20% of coefficient of variation for repeatability, intraday and interday precision analysis, respectively, indicating a good stability of the method developed. 113 metabolites were identified in cured tobacco based on accurate mass, retention time, and ms/ms fragments. the developed method was applied to a metabolomics study of cured tobacco from three growing regions. forty three metabolites were found to be contributed to the classification. it is shown that the developed method can be applied to metabolomics analysis of plant materials. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | plant metabolomics ; functional genomics ; quality-control ; gc-ms ; leaves ; metabolites ; extraction ; strategies ; separation ; database |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335196500003 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145646]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lili,Zhao, Chunxia,Chang, Yuwei,et al. Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions[J]. journal of separation science,2014,37(9-10):1067-1074. |
| APA | Li, Lili.,Zhao, Chunxia.,Chang, Yuwei.,Lu, Xin.,Zhang, Junjie.,...&Xu, Guowang.(2014).Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions.journal of separation science,37(9-10),1067-1074. |
| MLA | Li, Lili,et al."Metabolomics study of cured tobacco using liquid chromatography with mass spectrometry: Method development and its application in investigating the chemical differences of tobacco from three growing regions".journal of separation science 37.9-10(2014):1067-1074. |
入库方式： OAI收割
来源：大连化学物理研究所
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