Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification
文献类型：期刊论文
| 作者 | Hu, Lianghai1,2; Boos, Karl-Siegfried3; Ye, Mingliang2; Zou, Hanfa2 |
| 刊名 | talanta
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 127页码:191-195 |
| 关键词 | Restrict-access materials Sample extraction Proteomics Serum peptides Two-dimensional |
| 英文摘要 | the selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (scx-ram) coupled with two-dimensional rp-rp liquid chromatography mass spectrometry was developed. the operation of the on-line extraction system is simple to use and does not need complex equipments. the two-dimensional rp-rp was proved to be orthogonal and efficient to separate peptides extracted from human serum. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | multidimensional liquid-chromatography ; rp-rp-hplc ; sample preparation ; selective extraction ; mass-spectrometry ; biological-fluids ; lc-ms/ms ; peptidomics ; separation ; nanoparticles |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338620300026 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145700]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Hosp Grosshadem, Inst Clin Chem, Lab BioSeparat, D-81366 Munich, Germany |
| 推荐引用方式 GB/T 7714 | Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,et al. Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification[J]. talanta,2014,127:191-195. |
| APA | Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,&Zou, Hanfa.(2014).Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification.talanta,127,191-195. |
| MLA | Hu, Lianghai,et al."Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification".talanta 127(2014):191-195. |
入库方式： OAI收割
来源：大连化学物理研究所
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