OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC
文献类型：期刊论文
| 作者 | Dang, Jun1,2; Chen, Chen2; Shao, Yun2; Mei, Lijuan2; Zhang, Huaigang2; Zhong, Zhengsheng3; Wang, Qilan2; Tao, Yanduo2 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2014-08-27 |
| 卷号 | 37期号:14页码:1940-1952 |
| 关键词 | peak area extraction process Gentiana Straminea Maxim. gentiopicroside response surface methodology HPLC |
| 英文摘要 | extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (rsm) was used to study the optimum extraction technology of gentiopicroside from gentiana straminea maxim. the analysis was performed on a high performance liquid chromatography (hplc) system with gentiopicroside peak areas as response values. a ternary quadratic polynomial model with good correlation (r-2=0.9786) was constructed and employed to guide the gentiopicroside extraction process. the optimum extraction conditions were an optimized extract time of 79.4min, a ratio of methanol aqueous solution to material weight of 23.7ml/g, methanol concentration of 92.6%, and three times extraction. under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3mg center dot g(-1). through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | assisted extraction ; secoiridoids ; macrophylla |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334067600003 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145761]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dang, Jun,Chen, Chen,Shao, Yun,et al. OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC[J]. journal of liquid chromatography & related technologies,2014,37(14):1940-1952. |
| APA | Dang, Jun.,Chen, Chen.,Shao, Yun.,Mei, Lijuan.,Zhang, Huaigang.,...&Tao, Yanduo.(2014).OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC.journal of liquid chromatography & related technologies,37(14),1940-1952. |
| MLA | Dang, Jun,et al."OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC".journal of liquid chromatography & related technologies 37.14(2014):1940-1952. |
入库方式： OAI收割
来源：大连化学物理研究所
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