Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs
文献类型：期刊论文
| 作者 | Hou, Shuhua1,2,3; Ji, Debin1,2; Liu, Wujun1,2; Wang, Lei1,2; Zhao, Zongbao (Kent)1,2 |
| 刊名 | bioorganic & medicinal chemistry letters
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 24期号:5页码:1307-1309 |
| 关键词 | Cofactor Malic enzyme Nicotinamide adenine dinucleotide Orthogonal redox system Enzyme kinetics |
| 英文摘要 | an activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (nad) analogs and malic enzyme (me) mutants identified some mutants capable of taking nad analogs as the cofactor. one particular pair, me-l310k/l404s and the analog b-8 had good catalytic efficiency and cofactor specificity. the new system gained about 1200-fold cofactor specificity shift from nad toward b-8 in terms of oxidative decarboxylation of l-malate. our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (c) 2014 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | chemistry, medicinal ; chemistry, organic |
| 研究领域[WOS] | pharmacology & pharmacy ; chemistry |
| 关键词[WOS] | bioorthogonal chemistry ; small molecules ; nad analogs ; discrimination ; dehydrogenase ; specificity ; systems ; life |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331705200012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145771]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Bohai Univ, Dept Chem, Liaoning Key Lab Synth & Applicat Funct Cpds, Jinzhou 121013, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Shuhua,Ji, Debin,Liu, Wujun,et al. Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs[J]. bioorganic & medicinal chemistry letters,2014,24(5):1307-1309. |
| APA | Hou, Shuhua,Ji, Debin,Liu, Wujun,Wang, Lei,&Zhao, Zongbao .(2014).Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs.bioorganic & medicinal chemistry letters,24(5),1307-1309. |
| MLA | Hou, Shuhua,et al."Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs".bioorganic & medicinal chemistry letters 24.5(2014):1307-1309. |
入库方式： OAI收割
来源：大连化学物理研究所
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