A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells
文献类型：期刊论文
| 作者 | Liu, Zhao-Ming1,4; Feng, Lei2; Hou, Jie3; Lv, Xia1; Ning, Jing1,3; Ge, Guang-Bo1; Wang, Ke-Wei2; Cui, Jing-Nan2; Yang, Ling1 |
| 刊名 | sensors and actuators b-chemical
 |
| 出版日期 | 2014-12-15 |
| 卷号 | 205页码:151-157 |
| 关键词 | Human carboxylesterase 2 Ratiometric fluorescent probe 4-Hydroxy-N-butyl-1 Bioimaging 8-naphthalimide |
| 英文摘要 | a new ratiometric fluorescent probe derived from 4-hydroxy-n-butyl-1,8-naphthalimide (hnn) has been developed for selective detection of human carboxylesterase 2 (hce2). the probe is designed by introducing benzoyl moiety to hnn, based on the intramolecular charge transfer (ict) mechanism. the probe displays satisfying stability under physiological ph conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hce2 and release of hnn which leads to a remarkable red shift in emission spectra (148 nm). the newly designed probe exhibits excellent selectivity towards hce2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hce2 in human living cells, have also been demonstrated. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, analytical ; electrochemistry ; instruments & instrumentation |
| 研究领域[WOS] | chemistry ; electrochemistry ; instruments & instrumentation |
| 关键词[WOS] | human intestinal carboxylesterase ; mammalian carboxylesterases ; human liver ; catalytic-properties ; cpt-11 hydrolysis ; tumor-tissue ; probe ; irinotecan ; inhibitors ; metabolism |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343117600020 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145788]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116024, Peoples R China 2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 3.Dalian Med Univ, Dalian 116044, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhao-Ming,Feng, Lei,Hou, Jie,et al. A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells[J]. sensors and actuators b-chemical,2014,205:151-157. |
| APA | Liu, Zhao-Ming.,Feng, Lei.,Hou, Jie.,Lv, Xia.,Ning, Jing.,...&Yang, Ling.(2014).A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells.sensors and actuators b-chemical,205,151-157. |
| MLA | Liu, Zhao-Ming,et al."A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells".sensors and actuators b-chemical 205(2014):151-157. |
入库方式： OAI收割
来源：大连化学物理研究所
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