Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins
文献类型：期刊论文
| 作者 | Lu, Congcong1,2; Zhang, Kai1,2,3,4; Zhang, Yi5,6; Tan, Minjia5,6; Li, Yijun1,2; He, Xiwen1,2; Zhang, Yukui1,2,7 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2014-12-12 |
| 卷号 | 1372页码:34-41 |
| 关键词 | Proteomics HPLC-MS/MS Chemical probe Target proteins Vorinostat |
| 英文摘要 | inhibitors of histone deacetylases (hdacs) have been considered to be new anticancer agents. as a key inhibitor of hdac, vorinostat can cause growth arrest and death of a broad of transformed cells and interact with a variety of substrates. a comprehensive analysis of proteins interacting with hdac inhibitors is of great importance in understanding molecular mechanisms of the drugs. here, we reported the preparation and characterization of vorinostat-coated beads for profiling of novel target proteins of vorinostat (a key hdac inhibitor). the enriched proteins were further analyzed by hplc-ms/ms. besides the known substrates, there were also several novel enriched protein candidates, one of which was a metalloenzyme a-enolase (eno-1). according to our best knowledge, it is the first time that eno-1 has been detected as a potential target of vorinostat through chemoproteomics approach. further competition analysis indicated that eno-1 may be co-enriched as a substrate complex. our results demonstrated that the chemical probe combined with proteomics approach may be developed as a potential tool to identify target proteins of drugs. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | histone deacetylase inhibitors ; lysine acetylation ; molecular-mechanisms ; hydroxamic acid ; cancer cells ; hdac ; saha ; therapies ; complexes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347020900004 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145808]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 3.Tianjin Med Univ, Basic Med Sch, Dept Biochem & Mol Biol, Tianjin 300070, Peoples R China 4.Tianjin Med Univ, Basic Med Sch, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 201203, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 7.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Congcong,Zhang, Kai,Zhang, Yi,et al. Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins[J]. journal of chromatography a,2014,1372:34-41. |
| APA | Lu, Congcong.,Zhang, Kai.,Zhang, Yi.,Tan, Minjia.,Li, Yijun.,...&Zhang, Yukui.(2014).Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins.journal of chromatography a,1372,34-41. |
| MLA | Lu, Congcong,et al."Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins".journal of chromatography a 1372(2014):34-41. |
入库方式： OAI收割
来源：大连化学物理研究所
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