Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae
文献类型：期刊论文
| 作者 | Zhao, Pengchao1,2; Quan, Chunshan3; Wang, Yingguo1,2; Wang, Jianhua1,2; Fan, Shengdi3 |
| 刊名 | journal of basic microbiology
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 54期号:5页码:448-456 |
| 关键词 | Gene clusters Lipopeptides Fungistatic agent Fengycin A Antifungal mechanism |
| 英文摘要 | in recent years, bacillus species have received considerable attention for the biological control of many fungal diseases. in this study, bacillus amyloliquefaciens q-426 was tested for its potential use against a variety of plant pathogens. our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the q-426 genome. lipopeptides such as bacillomycin d, fengycin a, and fengycin b were purified from the bacterial culture broth and subsequently identified by esi-mass spectrometry. the minimal inhibitory concentration of fengycin a against fusarium oxysporum f. sp. spinaciae w.c. snyder & h.n. hansen o-27 was determined to be 31.25gml(-1). however, exposure of fungal cells to 50gml(-1) of fengycin a did not allow permeation of fluorescein diacetate into the cytoplasm through the cell membrane. moreover, leakage of intracellular inorganic cations, nucleic acid and protein were also not detected, indicating that the fungal cell membrane is not the primary target of action for fengycin a. profound morphological changes were observed in the f. oxysporum strain and spore germination was completely inhibited, suggesting that 50gml(-1) of fengycin a acts, at least, as a fungistatic agent. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | microbiology |
| 研究领域[WOS] | microbiology |
| 关键词[WOS] | nonribosomal synthesis ; antifungal activity ; iturin-a ; subtilis ; lipopeptides ; surfactin ; fengycin ; mechanism ; reveals ; soil |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336216700013 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145837]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Nationalities Univ, Dept Life Sci, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Pengchao,Quan, Chunshan,Wang, Yingguo,et al. Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae[J]. journal of basic microbiology,2014,54(5):448-456. |
| APA | Zhao, Pengchao,Quan, Chunshan,Wang, Yingguo,Wang, Jianhua,&Fan, Shengdi.(2014).Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae.journal of basic microbiology,54(5),448-456. |
| MLA | Zhao, Pengchao,et al."Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp spinaciae".journal of basic microbiology 54.5(2014):448-456. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。