Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method
文献类型：期刊论文
| 作者 | Zhao, Yanni; Zhao, Chunxia; Li, Yanli; Chang, Yuwei; Zhang, Junjie; Zeng, Zhongda; Lu, Xin; Xu, Guowang |
| 刊名 | journal of separation science
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 37期号:16页码:2177-2184 |
| 关键词 | Flue-cured tobacco Gas chromatography with mass spectrometry Pseudotargeted analysis Selected-ion monitoring |
| 英文摘要 | a pseudotargeted method based on gas chromatography and mass spectrometry with selected-ion monitoring was established to investigate the metabolite differences of flue-cured tobacco from three different growing regions. the mixed solvent of acetonitrile/isopropanol/water (3:3:2, v/v/v) was chosen as the optimal extraction system based on the good repeatability and extraction efficiency. a self-developed software coupled with commercial software was used to establish the pseudotargeted method including 289 peaks and 47 groups. multivariable statistical analysis indicated that tobacco samples can be obviously separated based on the geographical origins. on the basis of a mann-whitney u test, organic acids, phenols, and alkaloids had higher levels in hunan province. in contrast, a large proportion of amino acids (including l-tyrosine, l-proline, and serine), sucrose, and linoleic acid were the highest in yunnan province. meanwhile, multiple metabolic pathways (including carbohydrate metabolism, tricarboxylic acid cycle, and nitrogen metabolism) were influenced by growing regions. twenty-eight differential metabolites, which had great contributions to the classification of tobacco samples of three growing regions, were further defined. the results demonstrated that the developed pseudotargeted method was a powerful tool to investigate the metabolic profiling of tobacco leaves and discriminate tobacco leaves of different growing regions. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; volatile organic-acids ; gc-ms ; transgenic rice ; quality-control ; wild-type ; reveals ; leaves ; arabidopsis ; responses |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340556300012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145840]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yanni,Zhao, Chunxia,Li, Yanli,et al. Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method[J]. journal of separation science,2014,37(16):2177-2184. |
| APA | Zhao, Yanni.,Zhao, Chunxia.,Li, Yanli.,Chang, Yuwei.,Zhang, Junjie.,...&Xu, Guowang.(2014).Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method.journal of separation science,37(16),2177-2184. |
| MLA | Zhao, Yanni,et al."Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method".journal of separation science 37.16(2014):2177-2184. |
入库方式： OAI收割
来源：大连化学物理研究所
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