Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides
文献类型：期刊论文
| 作者 | Jiang, Maorong1; Guo, Zhimou2; Wang, Caiping1; Yang, Yuming1; Liang, Xinmiao2; Ding, Fei1 |
| 刊名 | neuroscience letters
 |
| 出版日期 | 2014-10-03 |
| 卷号 | 581页码:32-36 |
| 关键词 | Chitooligosaccharides Chito-oligomer Schwann cell Dorsal root ganglion (DRG) Neurite outgrowth |
| 英文摘要 | chitooligosaccharides (coss) are obtained from the in vivo and in vitro degradation of chitosan, which is a natural biomaterial used successfully for peripheral nerve repair. also, coss have been reported to be beneficial for the nervous system and especially able to promote peripheral nerve regeneration. to determine which component in a mixture of coss was really responsible for the neurotrophic action of coss, this study was performed to separate the active components of coss and compare the neural activity of different pure components. hydrophilic interaction liquid chromatography (hilic) was used to separate 5 chito-oligomers with single degrees of polymerization (dps) from a mixture of coss. they were chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose with dps of 2-6, respectively. mtt assay indicated that chitotriose induced the greatest increase in schwann cell survival among 5 chito-oligomers. immunocytochemistry with anti-nf-200 showed that chitotriose significantly encouraged neurite outgrowth from dorsal root ganglion (drg) explants with the greatest effect among 5 chito-oligomers. our results suggest that chitotriose may be a key component in coss mixture. (c) 2014 elsevier ireland ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | neurosciences |
| 研究领域[WOS] | neurosciences & neurology |
| 关键词[WOS] | molecular-weight chitosan ; neurite outgrowth ; nerve grafts ; in-vitro ; neurons ; cells ; acid ; regeneration ; pathway ; cos |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342872500006 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145871]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nantong Univ, Jiangsu Key Lab Neuroregenerat, Nantong 226001, JS, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, LN, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Maorong,Guo, Zhimou,Wang, Caiping,et al. Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides[J]. neuroscience letters,2014,581:32-36. |
| APA | Jiang, Maorong,Guo, Zhimou,Wang, Caiping,Yang, Yuming,Liang, Xinmiao,&Ding, Fei.(2014).Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides.neuroscience letters,581,32-36. |
| MLA | Jiang, Maorong,et al."Neural activity analysis of pure chito-oligomer components separated from a mixture of chitooligosaccharides".neuroscience letters 581(2014):32-36. |
入库方式： OAI收割
来源：大连化学物理研究所
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