Evaluation of the peak variance in gradient liquid chromatography
文献类型：期刊论文
| 作者 | Hao, Weiqiang1,2; Di, Bin3; Chen, Qiang1; Wang, Junde4; Yang, Yongbing1; Yue, Bangyi1 |
| 刊名 | analyst
 |
| 出版日期 | 2014 |
| 卷号 | 139期号:6页码:1504-1511 |
| 英文摘要 | the peak variance obtained under single stepwise (ss), single linear (sl) and ladder-like (ll) gradient conditions was evaluated by taking into account the variation of plate height with mobile phase composition. lc tests were performed on a c18 column using a mixture of methanol and water as the mobile phase and six aromatic compounds (anisole, o-cresol, biphenyl, phenol, aniline and acetophenone) as the model samples. it was found that the relationship between the retention factor and mobile phase composition could be described by the linear solvent strength model. the plate height that was measured under isocratic conditions varied with mobile phase composition. this variation could be described by a quadratic polynomial in this work. under gradient conditions, the experimental and predicted values of peak variance were found to be well consistent. the discrepancy between the experimental and predicted values in the ss gradient was larger than that in the sl or ll gradient. this may be ascribed to the distortion of the gradient profile as a result of dispersion that occurred in the mobile phase. by using the dispersive gradient profile that was obtained at the column inlet for the calculation, the discrepancy between the experimental and predicted values in the ss gradient may be minimized. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-transfer ; elution chromatography ; compression factor ; organic modifier ; model ; retention ; optimization ; bandwidth ; predict ; width |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331867500031 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145894]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nanjing Univ, High Tech Res Inst, Changzhou 213164, Peoples R China 2.Shanghai Tobacco Grp Co LTD, Shanghai Tobacco Grp, CNTC, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China 3.China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing 210009, Jiangsu, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Weiqiang,Di, Bin,Chen, Qiang,et al. Evaluation of the peak variance in gradient liquid chromatography[J]. analyst,2014,139(6):1504-1511. |
| APA | Hao, Weiqiang,Di, Bin,Chen, Qiang,Wang, Junde,Yang, Yongbing,&Yue, Bangyi.(2014).Evaluation of the peak variance in gradient liquid chromatography.analyst,139(6),1504-1511. |
| MLA | Hao, Weiqiang,et al."Evaluation of the peak variance in gradient liquid chromatography".analyst 139.6(2014):1504-1511. |
入库方式： OAI收割
来源：大连化学物理研究所
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