Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres
文献类型：期刊论文
| 作者 | Deng, Nan1,2; Zhu, Guijie1; Chen, Yuanbo1,2; Wu, Qi1,2; Liang, Zhen1; Sui, Zhigang1; Zhang, Lihua1; Yang, Kaiguang1; Zhang, Yukui1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2014-05-15 |
| 卷号 | 826页码:43-50 |
| 关键词 | Ampholine Fractionation Human plasma Protein dynamic range |
| 英文摘要 | a novel protein sample pretreatment method based on ampholine immobilized polymer microsphere (ampholine@pm) was developed for the fractionation of intact proteins prior to protein digestion and peptide analysis to reduce the dynamic range of human plasma proteome. after incubation with our prepared ampholine@pm, the captured plasma proteins were successively desorbed by 2 m nacl, 100 mm glycine-hydrochloric acid, and 30% (v/v) acetonitrile with 0.1% (v/v) trifluoroacetic acid. the sds-page results showed the protein dynamic range in such three fractions was obviously reduced as compared with the native plasma. on-particle digestion was ultimately performed to release all proteins retained on ampholine@pm. followed by mupit analysis, the number of identified proteins in plasma was improved by 75% after ampholine@pm treatment. furthermore, the spectral count of 9 high abundance proteins was decreased by 37.6-97.2%, and the identified low abundance protein (< 100 ng ml(-1)) number was increased from 4 to 17. these results demonstrated that the fractionation by ampholine@pm could efficiently decrease the protein dynamic range in abundance, beneficial to achieve the deep coverage identification of human plasma proteome. (c) 2014 elsevier b. v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | multidimensional liquid-chromatography ; lectin affinity-chromatography ; tandem mass-spectrometry ; ligand library treatment ; human serum proteome ; exchange chromatography ; biomarker discovery ; gel electrophoresis ; peptide libraries ; reversed-phase |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335452300006 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145916]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Nan,Zhu, Guijie,Chen, Yuanbo,et al. Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres[J]. analytica chimica acta,2014,826:43-50. |
| APA | Deng, Nan.,Zhu, Guijie.,Chen, Yuanbo.,Wu, Qi.,Liang, Zhen.,...&Zhang, Yukui.(2014).Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres.analytica chimica acta,826,43-50. |
| MLA | Deng, Nan,et al."Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres".analytica chimica acta 826(2014):43-50. |
入库方式： OAI收割
来源：大连化学物理研究所
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