Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp.
文献类型：期刊论文
| 作者 | Zhu, Ben-Wei1,2; Huang, Li-Shu-Xin1,2; Tan, Hai-Dong1; Qin, Yu-Qi3; Du, Yu-Guang1,4; Yin, Heng1 |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 37期号:2页码:409-415 |
| 关键词 | Alginate lyase Oligosaccharides Poly-beta-D-mannuronate lyase Pseudomonas mendocina |
| 英文摘要 | an alginate lyase gene, alga, encoding a new poly beta-d-mannuronate (polym)-specific alginate lyase alga, was cloned from pseudomonas sp. e03. the recombinant alga with (his)(6)-tag, consisting of 364 amino acids (40.4 kda),was purified using ni-nta sepharose. the purified lyase had maximal activity (222 eu/mg) at ph 8 and 30 a degrees c and also maintained activity between ph 7-9 and below 45 a degrees c. it exclusively and endolytically depolymerized polym by beta-elimination into oligosaccharides with degrees of polymerization (dp) of 2-5. due to its high substrate specificity, alga could be a valuable tool for production of polym oligosaccharides with low dp and for determining the fine structure of alginate. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | raw264.7 cells ; oligosaccharides ; purification ; mannuronate ; guluronate ; induction ; linkages |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349230800020 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145976]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Nat Prod & Glycobiotechnol Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Ben-Wei,Huang, Li-Shu-Xin,Tan, Hai-Dong,et al. Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp.[J]. biotechnology letters,2015,37(2):409-415. |
| APA | Zhu, Ben-Wei,Huang, Li-Shu-Xin,Tan, Hai-Dong,Qin, Yu-Qi,Du, Yu-Guang,&Yin, Heng.(2015).Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp..biotechnology letters,37(2),409-415. |
| MLA | Zhu, Ben-Wei,et al."Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp.".biotechnology letters 37.2(2015):409-415. |
入库方式： OAI收割
来源：大连化学物理研究所
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