Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction
文献类型：期刊论文
| 作者 | Li, Yinghao1,2; Jia, Guoqing1; Wang, Changhao1,2; Cheng, Mingpan1,2; Li, Can1 |
| 刊名 | chembiochem
 |
| 出版日期 | 2015-03-02 |
| 卷号 | 16期号:4页码:618-624 |
| 关键词 | Diels-Alder reaction DNA enantioselective catalysis G-quadruplexes human telomeric DNA metalloenzyme |
| 英文摘要 | short human telomeric (ht) dna sequences form single g-quadruplex (g(4)) units and exhibit structure-based stereocontrol for a series of reactions. however, for more biologically relevant higher-order ht g(4)-dnas (beyond a single g(4) unit), the catalytic performances are unknown. here, we found that higher-order ht g(4)-dna copper metalloenzymes (two or three g(4) units) afford remarkably higher enantioselectivity (>90% ee) and a five- to sixfold rate increase, compared to a single g(4) unit, for the diels-alder reaction. electron paramagnetic resonance (epr) and enzymatic kinetic studies revealed that the distinct catalytic function between single and higher-order g(4)-dna copper metalloenzymes can be attributed to different cu-ii coordination environments and substrate specificity. our finding suggests that, like protein enzymes and ribozymes, higher-order structural organization is crucial for g(4)-dna-based catalysis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; chemistry, medicinal |
| 研究领域[WOS] | biochemistry & molecular biology ; pharmacology & pharmacy |
| 关键词[WOS] | asymmetric catalysis ; k+ solution ; circular-dichroism ; binding-site ; cd spectra ; ribozyme ; water ; polymorphism ; stability ; multimers |
| 收录类别 | SCI ; IC |
| 语种 | 英语 |
| WOS记录号 | WOS:000350138700012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145978]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yinghao,Jia, Guoqing,Wang, Changhao,et al. Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction[J]. chembiochem,2015,16(4):618-624. |
| APA | Li, Yinghao,Jia, Guoqing,Wang, Changhao,Cheng, Mingpan,&Li, Can.(2015).Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction.chembiochem,16(4),618-624. |
| MLA | Li, Yinghao,et al."Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction".chembiochem 16.4(2015):618-624. |
入库方式： OAI收割
来源：大连化学物理研究所
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