Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism
文献类型：期刊论文
| 作者 | Liu, Yinghui1; Feng, Yanbin1; Wang, Yayue1,2; Li, Xia3; Cao, Xupeng1; Xue, Song1 |
| 刊名 | biochemical and biophysical research communications
 |
| 出版日期 | 2015-02-13 |
| 卷号 | 457期号:3页码:398-403 |
| 关键词 | MCAT Synechocystis sp PCC 6803 Structure Intermediate |
| 英文摘要 | malonyl-coenzyme a: acyl-carrier protein transacylase (mcat) catalyzes the transfer of malonyl group from malonyl-coa to the holo-acyl carrier protein (holo-acp), yielding malonyl-acr the overall reaction has been extensively studied in heterotrophic microorganisms, while its mechanism in photosynthetic autotrophs as well as the stepwise reaction information remains unclear. here the 2.42 angstrom crystal structure of mcat from photosynthetic microorganism synechocystis sp. pcc 6803 is presented. it demonstrates that arg113, ser88 and his188 constitute catalytic triad. the second step involved acpmcat-malonyl intermediate is speed-limited instead of the malonyl-coa-mcat intermediate in the first step. therefore his87, arg113 and ser88 render different contributions for the two intermediates. additionally, s88t mutant initializes the reaction by h87 deprotonating s88t which is different from the wild type. (c) 2015 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biophysics |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics |
| 关键词[WOS] | acyl carrier protein ; malonyl-coa ; crystal-structure ; escherichia-coli ; streptomyces-coelicolor ; helicobacter-pylori ; transacylase ; biosynthesis ; specificity ; resolution |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351254700027 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146087]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Dalian Polytech Univ, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yinghui,Feng, Yanbin,Wang, Yayue,et al. Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism[J]. biochemical and biophysical research communications,2015,457(3):398-403. |
| APA | Liu, Yinghui,Feng, Yanbin,Wang, Yayue,Li, Xia,Cao, Xupeng,&Xue, Song.(2015).Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism.biochemical and biophysical research communications,457(3),398-403. |
| MLA | Liu, Yinghui,et al."Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp PCC 6803 reveal its stepwise catalytic mechanism".biochemical and biophysical research communications 457.3(2015):398-403. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。