BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum
文献类型：期刊论文
| 作者 | Jia, Ming-Yan1,3; Niu, Li-Ya2; Zhang, Yu1; Yang, Qing-Zheng2; Tung, Chen-Ho2; Guan, Ya-Feng1; Feng, Liang1 |
| 刊名 | acs applied materials & interfaces
 |
| 出版日期 | 2015-03-18 |
| 卷号 | 7期号:10页码:5907-5914 |
| 关键词 | BODIPY cysteine homocysteine glutathione serum |
| 英文摘要 | cysteine (cys), homocysteine (hey), and glutathione (gsh) are interconbected and play essential roles for regulating the redox balance of biological processes. however, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. in this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (bodipy)-based fluorometric sensor. at a specified period of time, after reacting with excess sensor, hcy and gsh form predominantly sulfur-substituted bodipy, while cys generates sulfur-amino-dibodipy due to a fast substitution-rearrangement substitution reaction. a significant difference in polarities of these respective major products simplifies their separation by tlc, thus leading to the simultaneous determination of cys, hey, and gsh readily. the sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (hplc). |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | nanoscience & nanotechnology ; materials science, multidisciplinary |
| 研究领域[WOS] | science & technology - other topics ; materials science |
| 关键词[WOS] | ratiometric fluorescent sensor ; different emission channels ; highly selective detection ; living cells ; glutathione-reductase ; total homocysteine ; total cysteine ; probe ; plasma ; thiols |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351420300034 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146089]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100191, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Ming-Yan,Niu, Li-Ya,Zhang, Yu,et al. BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum[J]. acs applied materials & interfaces,2015,7(10):5907-5914. |
| APA | Jia, Ming-Yan.,Niu, Li-Ya.,Zhang, Yu.,Yang, Qing-Zheng.,Tung, Chen-Ho.,...&Feng, Liang.(2015).BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum.acs applied materials & interfaces,7(10),5907-5914. |
| MLA | Jia, Ming-Yan,et al."BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum".acs applied materials & interfaces 7.10(2015):5907-5914. |
入库方式： OAI收割
来源：大连化学物理研究所
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