Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases
文献类型：期刊论文
| 作者 | Wang, Yayue1,2; Xin, Yanjuan1; Cao, Xupeng1; Xue, Song1 |
| 刊名 | world journal of microbiology & biotechnology
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 31期号:4页码:669-673 |
| 关键词 | Pseudomonas stutzeri Inducers 2-Haloacid dehalogenase Production |
| 英文摘要 | there was no direct correlation in substrate specificity between the metabolism of pseudomonas stutzeri deh138 and its corresponding dehalogenase. dehalogenase substrates that could be dehalogenated might not be degraded by deh138 or vice versa. basing on this, different approaches to enhance l-2-haloacid dehalogenase (l-dex) production in deh138 via the combination of non-halogenated compounds with different inducers were applied. the optimum approach to obtain more l-dex from deh138 was the combination of dl-lactate and dl-2-chlorobutyrate, with 5.7-fold greater production and 11.7-fold greater productivity of the enzyme after optimization. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | sponge hymeniacidon-perlevis ; 2-haloacid dehalogenase ; 1,2-dichloroethane ; purification ; degradation ; acids |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351294200012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146099]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yayue,Xin, Yanjuan,Cao, Xupeng,et al. Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases[J]. world journal of microbiology & biotechnology,2015,31(4):669-673. |
| APA | Wang, Yayue,Xin, Yanjuan,Cao, Xupeng,&Xue, Song.(2015).Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases.world journal of microbiology & biotechnology,31(4),669-673. |
| MLA | Wang, Yayue,et al."Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases".world journal of microbiology & biotechnology 31.4(2015):669-673. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。