Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix
文献类型：期刊论文
| 作者 | Zhao Yanyan1; Wang Liheng2; Guo Zhimou3; Chi Xiaofei2; Ma Xiaochi1; Qi Yan1; Fang Shinong1; Li Xiuling3; Liang Xinmiao3 |
| 刊名 | chemical research in chinese universities
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 31期号:1页码:44-52 |
| 关键词 | Click OEG-CD[olio(ethylene glycol)(OEG) linked beta-cyclodextrin(CD)] matrix Multi-phosphopeptide enrichment MS detection |
| 英文摘要 | detection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. click oeg-cd[olio(ethylene glycol)(oeg) linked,beta-cyclodextrin(cd)] matrix has shown excellent performance in phosphopeptide enrichment. however, few multi-phosphopeptides were detected previously. in this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(ms) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. mass spectra were obtained on a nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (lc-esi-qtoe-ms). the enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion a-casein as test sample. when the loading amount was 573 pmol/mg matrix, up to 20 a-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. the result is much better than that of our previous report. the reduction of ion-suppression effect arised from the existence of high-abundance non- or mono-phosphopeptides and the stronger interactions between multi-phosphopepides and the matrix were contributed to the result. the study could be helpful to the better utilization of click oeg,cd matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | phosphoproteome analysis ; phosphorylation analysis ; protein-phosphorylation ; selective enrichment ; chromatography ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351479100009 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146104]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China 2.Second Hosp Dalian City, Dalian 116011, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Yanyan,Wang Liheng,Guo Zhimou,et al. Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix[J]. chemical research in chinese universities,2015,31(1):44-52. |
| APA | Zhao Yanyan.,Wang Liheng.,Guo Zhimou.,Chi Xiaofei.,Ma Xiaochi.,...&Liang Xinmiao.(2015).Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix.chemical research in chinese universities,31(1),44-52. |
| MLA | Zhao Yanyan,et al."Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix".chemical research in chinese universities 31.1(2015):44-52. |
入库方式： OAI收割
来源：大连化学物理研究所
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