Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography
文献类型：期刊论文
| 作者 | Liang, Tu1; Fu, Qing1; Shen, Aijin2; Wang, Hui1; Jin, Yu1; Xin, Huaxia1; Ke, Yanxiong1; Guo, Zhimou2; Liang, Xinmiao1,2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2015-04-03 |
| 卷号 | 1388页码:110-118 |
| 关键词 | Rebaudioside A Stationary phase Hydrophilic interaction liquid chromatography (HILIC) Mixed-mode Thiol-ene click chemistry |
| 英文摘要 | a diterpene glycoside compound, rebaudioside a (commonly abbreviated as ra), was immobilized onto porous silica surface through "thiol-ene" click chemistry strategy. the successful immobilization of the ra on the silica support was confirmed by ft-ir and elemental analysis. chromatographic characteristics of the new stationary phase, named click te-ra, were evaluated by a set of diverse analytes such as carbohydrates, nucleosides, and organic acids in hydrophilic interaction liquid chromatography (hilic) mode. the effects of water content, buffer ph and concentration were investigated and a typical hilic retention feature of click te-ra was observed at high organic modifier content. the click te-ra stationary phase was further studied by a series of glycoside compounds. tunable retention mechanisms from hydrophilic to hydrophobic interactions were observed. separation of very polar compounds including oligosaccharides, nucleic acid bases and nucleosides using click te-ra in hilic mode was successfully accomplished. in addition, separation of saponins both in hilic and reversed-phase liquid chromatography (rplc) modes was performed, demonstrating the presence of orthogonality between two different modes on click te-ra column. the multiple interactions induced by polar sugar group and hydrophobic aglycone group allowed this click te-ra to serve as a multi-mode stationary phase in two-dimensional liquid chromatography. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | mixed-mode chromatography ; separation ; retention ; saccharides ; performance ; selectivity ; saponins ; behavior ; acids |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351792400013 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146125]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Tu,Fu, Qing,Shen, Aijin,et al. Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography[J]. journal of chromatography a,2015,1388:110-118. |
| APA | Liang, Tu.,Fu, Qing.,Shen, Aijin.,Wang, Hui.,Jin, Yu.,...&Liang, Xinmiao.(2015).Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography.journal of chromatography a,1388,110-118. |
| MLA | Liang, Tu,et al."Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography".journal of chromatography a 1388(2015):110-118. |
入库方式： OAI收割
来源：大连化学物理研究所
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