Identifying and applying a highly selective probe to simultaneously determine the O-glucuronidation activity of human UGT1A3 and UGT1A4
文献类型：期刊论文
| 作者 | Jiang, Li1; Liang, Si-Cheng2,3; Wang, Chao1; Ge, Guang-Bo2; Huo, Xiao-Kui1; Qi, Xiao-Yi4; Deng, Sa1; Liu, Ke-Xin1; Ma, Xiao-Chi1 |
| 刊名 | scientific reports
 |
| 出版日期 | 2015-04-17 |
| 卷号 | 5 |
| 英文摘要 | glucuronidation mediated by uridine 59-diphospho (udp)-glucuronosyltransferase is an important detoxification pathway. however, identifying a selective probe of udp-glucuronosyltransferase is complicated because of the significant overlapping substrate specificity displayed by the enzyme. in this paper, desacetylcinobufagin (dacb) 3-o- and 16-o-glucuronidation were found to be isoform-specific probe reactions for ugt1a4 and ugt1a3, respectively. dacb was well characterized as a probe for simultaneously determining the catalytic activities of o-glucuronidation mediated by ugt1a3 and ugt1a4 from various enzyme sources, through a sensitive analysis method. |
| WOS标题词 | science & technology |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | human udp-glucuronosyltransferases ; human liver-microsomes ; drug-glucuronidation ; n-glucuronidation ; mass-spectrometry ; chromatography ; substrate ; acid ; identification ; amitriptyline |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353275700001 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146159]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Acad Integrat Med, Coll Pharm, Key Lab Pharmacokinet & Drug Transport Liaoning, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Li,Liang, Si-Cheng,Wang, Chao,et al. Identifying and applying a highly selective probe to simultaneously determine the O-glucuronidation activity of human UGT1A3 and UGT1A4[J]. scientific reports,2015,5. |
| APA | Jiang, Li.,Liang, Si-Cheng.,Wang, Chao.,Ge, Guang-Bo.,Huo, Xiao-Kui.,...&Ma, Xiao-Chi.(2015).Identifying and applying a highly selective probe to simultaneously determine the O-glucuronidation activity of human UGT1A3 and UGT1A4.scientific reports,5. |
| MLA | Jiang, Li,et al."Identifying and applying a highly selective probe to simultaneously determine the O-glucuronidation activity of human UGT1A3 and UGT1A4".scientific reports 5(2015). |
入库方式： OAI收割
来源：大连化学物理研究所
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