Characterization of a new endo-type alginate lyase from Vibrio sp W13
文献类型：期刊论文
| 作者 | Zhu, Benwei1,2; Tan, Haidong1; Qin, Yuqi3; Xu, Qingsong1; Du, Yuguang1,4; Yin, Heng1 |
| 刊名 | international journal of biological macromolecules
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 75页码:330-337 |
| 关键词 | Alginate lyase Polysaccharide lyase family 7 Vibrio sp. |
| 英文摘要 | a gene, encoding a new alginate lyase algb, was identified and cloned from marine bacterium vibrio sp. w13. the recombinant alginate lyase was characterized followed by being purified on ni-nta sepharose. it exhibited the highest activity (457 u/mg) at ph 8.0 and 30 degrees c interestingly, algb possessed broader substrate specificity. it showed activities toward both polym (poly beta-d-mannuronate) and polyg (poly alpha-l-guluronate). furthermore, k-m values of algb toward alginate (0.67 mg/ml) and polymg (0.50 mg/ml) are lower than those toward polyg (1.04 mg/ml) and polym (6.90 mg/ml). the tlc and esi-ms analysis suggested that algb mainly released oligosaccharides with dp of 2-5 from the four kinds of substrates in an endolytic manner. therefore, it may be a potent tool to produce alginate oligosaccharides with low dp. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | oligoalginate lyase ; sequence-analysis ; saccharophagus degradans ; cystic-fibrosis ; raw264.7 cells ; cloning ; purification ; oligosaccharides ; polysaccharide ; depolymerization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352332700044 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146169]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Benwei,Tan, Haidong,Qin, Yuqi,et al. Characterization of a new endo-type alginate lyase from Vibrio sp W13[J]. international journal of biological macromolecules,2015,75:330-337. |
| APA | Zhu, Benwei,Tan, Haidong,Qin, Yuqi,Xu, Qingsong,Du, Yuguang,&Yin, Heng.(2015).Characterization of a new endo-type alginate lyase from Vibrio sp W13.international journal of biological macromolecules,75,330-337. |
| MLA | Zhu, Benwei,et al."Characterization of a new endo-type alginate lyase from Vibrio sp W13".international journal of biological macromolecules 75(2015):330-337. |
入库方式： OAI收割
来源：大连化学物理研究所
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