Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering
文献类型：期刊论文
| 作者 | Chen, Jun1,2; Yao, Mingdong1; Pagba, Cynthia V.3,4; Zheng, Yang1; Fei, Liping1; Feng, Zhaochi2; Barry, Bridgette A.3,4 |
| 刊名 | biochimica et biophysica acta-bioenergetics
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 1847期号:6-7页码:558-564 |
| 关键词 | Carbonyl bond Photosynthesis Proton transfer Electron transfer Lipid membrane Protein dynamics |
| 英文摘要 | in photosynthesis, photosystem ii (psii) harvests sunlight with bound pigments to oxidize water and reduce quinone to quinol, which serves as electron and proton mediators for solar-to-chemical energy conversion. at least two types of quinone cofactors in psii are redox-linked: q(a), and q(b). here, we for the first time apply 257-nm ultraviolet resonance raman (uvrr) spectroscopy to acquire the molecular vibrations of plastoquinone (pq) in psii membranes. owing to the resonance enhancement effect, the vibrational signal of pq in psii membranes is prominent. a strong band at 1661 cm(-1) is assigned to ring c=c/c=0 symmetric stretch mode (nu 8a mode) of pq and a weak band at 469 cm(-1) to ring stretch mode. by using a pump-probe difference uvrr method and a sample jet technique, the signals of qa and qb can be distinguished. a frequency difference of 1.4 cm(-1) in nu 8a vibrational mode between qa and qb is observed, corresponding to similar to 86 mv redox potential difference imposed by their protein environment. in addition, there are other pqs in the psii membranes. a negligible anharmonicity effect on their combination band at 2130 cm(-1) suggests that the 'other pqs' are situated in a hydrophobic environment. the detection of the 'other pqs' might be consistent with the view that another functional pq cofactor (not q(a) or q(b)) exists in psii. this uvrr approach will be useful to the study of quinone molecules in photosynthesis or other biological systems. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biophysics |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics |
| 关键词[WOS] | transform infrared-spectroscopy ; electron-transfer ; reaction centers ; spectra ; acceptor ; 1,4-benzoquinone ; reduction ; dynamics ; binding ; q(a) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355054900007 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146246]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 3.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA 4.Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA |
| 推荐引用方式 GB/T 7714 | Chen, Jun,Yao, Mingdong,Pagba, Cynthia V.,et al. Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering[J]. biochimica et biophysica acta-bioenergetics,2015,1847(6-7):558-564. |
| APA | Chen, Jun.,Yao, Mingdong.,Pagba, Cynthia V..,Zheng, Yang.,Fei, Liping.,...&Barry, Bridgette A..(2015).Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering.biochimica et biophysica acta-bioenergetics,1847(6-7),558-564. |
| MLA | Chen, Jun,et al."Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering".biochimica et biophysica acta-bioenergetics 1847.6-7(2015):558-564. |
入库方式： OAI收割
来源：大连化学物理研究所
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