Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor
文献类型：期刊论文
| 作者 | Wang, Yu1,2; Zhang, Ying1; Li, Na1,2; Chen, Li1,2; Zhang, Demeng1,2; Sun, Dongsheng1,2; Lv, Guojun1; Yu, Weiting1; Guo, Xin1; Ma, Xiaojun1 |
| 刊名 | bioprocess and biosystems engineering
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 38期号:7页码:1303-1312 |
| 关键词 | Cell culture Stirred tank bioreactor Microencapsulation Recombinant protein production |
| 英文摘要 | microencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. in this paper, we address the growth and desmodus rotundus salivary plasminogen activator (dspa) production of recombinant cho (rcho) in a stirred tank bioreactor. cells were cultured using two different methods-in an unmicroencapsulated versus microencapsulated culture-and compared differences between them in terms of cell reproduction and dspa protein productivity. compared to the unmicroencapsulated rcho, microencapsulated cells got higher cell density and prolonged the plateau phase. microencapsulated rcho promoted dspa production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of dspa was 3.3 higher than in unmicroencapsulated cells. negative relationship was found between specific growth rate and dspa production capacity of unit cells. these findings will facilitate the methods for higher dspa production in stirred tank bioreactors. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | biopharmaceutical benchmarks ; hepatocyte encapsulation ; cell-culture ; microcapsules ; stability ; oxygen ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355940900008 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146295]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Zhang, Ying,Li, Na,et al. Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor[J]. bioprocess and biosystems engineering,2015,38(7):1303-1312. |
| APA | Wang, Yu.,Zhang, Ying.,Li, Na.,Chen, Li.,Zhang, Demeng.,...&Ma, Xiaojun.(2015).Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor.bioprocess and biosystems engineering,38(7),1303-1312. |
| MLA | Wang, Yu,et al."Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor".bioprocess and biosystems engineering 38.7(2015):1303-1312. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。