Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Li, Lili; Lu, Xin; Zhao, Jieyu; Zhang, Junjie; Zhao, Yanni; Zhao, Chunxia; Xu, Guowang |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 407期号:17页码:5009-5020 |
| 关键词 | Lipidome Metabolome Fresh tobacco leaves Climatic factors Metabolomics |
| 英文摘要 | the combination of the lipidome and the metabolome can provide much more information in plant metabolomics studies. a method for the simultaneous extraction of the lipidome and the metabolome of fresh tobacco leaves was developed. method validation was performed on the basis of the optimal ratio of methanol to methyl tert-butyl ether to water (37:45:68) from the design of experiments. good repeatability was obtained. we found that 92.2 % and 91.6 % of the peaks for the lipidome and the metabolome were within a relative standard deviation of 20 %, accounting for 94.6 % and 94.6 % of the total abundance, respectively. the intraday and interday precisions were also satisfactory. a total of 230 metabolites, including 129 lipids, were identified. significant differences were found in lipidomic and metabolomic profiles of fresh tobacco leaves in different geographical regions. highly unsaturated galactolipids, phosphatidylethanolamines, predominant phosphatidylcholines, most of the polyphenols, amino acids, and polyamines had a higher content in yunnan province, and low-unsaturation-degree galactolipids, triacylglycerols, glucosylceramides with trihydroxy long-chain bases, acylated sterol glucosides, and some organic acids were more abundant in henan province. correlation analysis between differential metabolites and climatic factors indicated the vital importance of temperature. the fatty acid unsaturation degree of galactolipids could be influenced by temperature. accumulation of polyphenols and decreases in the ratios of stigmasterols to sitosterols and glucosylstigmasterols to glucosylsitosterols were also correlated with lower temperature in yunnan province. furthermore, lipids were more sensitive to climatic variations than other metabolites. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | tert-butyl ether ; cold-acclimation ; simultaneous extraction ; plant metabolomics ; gene-expression ; drought stress ; arabidopsis ; membrane ; reveals ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356461800012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146319]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lili,Lu, Xin,Zhao, Jieyu,et al. Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry[J]. analytical and bioanalytical chemistry,2015,407(17):5009-5020. |
| APA | Li, Lili.,Lu, Xin.,Zhao, Jieyu.,Zhang, Junjie.,Zhao, Yanni.,...&Xu, Guowang.(2015).Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry.analytical and bioanalytical chemistry,407(17),5009-5020. |
| MLA | Li, Lili,et al."Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry".analytical and bioanalytical chemistry 407.17(2015):5009-5020. |
入库方式： OAI收割
来源：大连化学物理研究所
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