Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study
文献类型：期刊论文
| 作者 | Liu, Xiaoyan1,2; Liu, Yanqiu3; Qu, Yang1; Cheng, Mengchun1; Xiao, Hongbin1,4 |
| 刊名 | toxicology research
 |
| 出版日期 | 2015 |
| 卷号 | 4期号:4页码:948-955 |
| 英文摘要 | emodin is one of the most representative natural anthraquinone polyphenols and the liver is one of the major target organs for drug-induced toxicology. the hepatocyte is frequently affected due to its role in emodin metabolism and accumulation. although the hepatotoxicity of emodin has been reported, its toxicological mechanism is still unclear. the purpose of the present study was to evaluate the cytotoxicity of emodin in cultured human normal liver cells (l-02), to investigate the toxicity-related metabolic pathways and to predict the possible toxicity mechanism. cell viability was analyzed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay. cytotoxicity tests demonstrated a concentration-dependent toxic effect of emodin on l-02 cells. cells were treated for 48 h with low, medium and high doses of emodin, respectively, and then subjected to metabolomics analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (uplc-ms). intracellular metabolomics analysis revealed that emodin significantly disturbed cellular glutathione and fatty acid metabolism. in addition, an emodin-cysteine adduct was identified in cell culture medium, and its level increased with increasing concentrations of emodin. the possible relationship among metabolic disorders, adduct formation and emodin hepatotoxicity was also discussed. this study provides new insight into the cytotoxicity of emodin on metabolic pathways in human liver cells. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | toxicology |
| 研究领域[WOS] | toxicology |
| 关键词[WOS] | fatty-acid oxidation ; chromatography-mass spectrometry ; aloe-emodin ; induced apoptosis ; l-02 cells ; induced hepatotoxicity ; gene-expression ; protein ; differentiation ; acetaminophen |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356612300017 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146324]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Coll Inst Integrat Med, Dalian 116044, Peoples R China 4.Beijing Univ Chinese Med, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoyan,Liu, Yanqiu,Qu, Yang,et al. Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study[J]. toxicology research,2015,4(4):948-955. |
| APA | Liu, Xiaoyan,Liu, Yanqiu,Qu, Yang,Cheng, Mengchun,&Xiao, Hongbin.(2015).Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study.toxicology research,4(4),948-955. |
| MLA | Liu, Xiaoyan,et al."Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study".toxicology research 4.4(2015):948-955. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。