Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
文献类型：期刊论文
| 作者 | Pan, Yangang1,2; Wang, Shaowen2,3; Shan, Yuping1,4,6; Zhang, Dinglin5; Gao, Jing1,2; Zhang, Min1,2; Liu, Shuheng1; Cai, Mingjun1; Xu, Haijiao1; Li, Guohui5 |
| 刊名 | small
 |
| 出版日期 | 2015-06-17 |
| 卷号 | 11期号:23页码:2782-2788 |
| 英文摘要 | the first step in most viral infections is the penetration of the cell membrane via endocytosis. however, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. here, the endocytosis of a single live virion (singapore grouper iridovirus, sgiv) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. for the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single sgiv by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pn, and the binding energy density increases with the engulfment depth. this report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, multidisciplinary ; chemistry, physical ; nanoscience & nanotechnology ; materials science, multidisciplinary ; physics, applied ; physics, condensed matter |
| 研究领域[WOS] | chemistry ; science & technology - other topics ; materials science ; physics |
| 关键词[WOS] | singapore grouper iridovirus ; individual influenza-viruses ; atomic-force microscopy ; host-cells ; proteomic analysis ; spotted grouper ; entry ; endocytosis ; macropinocytosis ; spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356448300011 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146335]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 4.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA 5.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China 6.Sch Chem & Life Sci, Adv Inst Mat Sci, Changchun 130012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Yangang,Wang, Shaowen,Shan, Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. small,2015,11(23):2782-2788. |
| APA | Pan, Yangang.,Wang, Shaowen.,Shan, Yuping.,Zhang, Dinglin.,Gao, Jing.,...&Wang, Hongda.(2015).Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane.small,11(23),2782-2788. |
| MLA | Pan, Yangang,et al."Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane".small 11.23(2015):2782-2788. |
入库方式： OAI收割
来源：大连化学物理研究所
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