Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis
文献类型：期刊论文
| 作者 | Zhang, Huiyan1,2; Zhao, Yong2; Cao, Hailong2; Mou, Guangqing1; Yin, Heng2 |
| 刊名 | international journal of biological macromolecules
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 79页码:72-75 |
| 关键词 | B. thuringiensis Lytic polysaccharide monooxygenases Polysaccharides |
| 英文摘要 | lytic polysaccharide monooxygenases (lpmos) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. despite the importance of lpmos in biomass conversion and the large number of lpmo genes in microorganisms, only a few lpmos have been well studied, and further characterization of these proteins is thus of interest. in this study, a chitin-active aa10 family lpmo from bacillus thuringiensis subsp. kurstaki, btlpmo10a, is described. this enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. these results provide new insights into the action mode of lpmos that may be affected by the substrates. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | cellulose ; protein ; enzymes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359166300010 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146448]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Huiyan,Zhao, Yong,Cao, Hailong,et al. Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis[J]. international journal of biological macromolecules,2015,79:72-75. |
| APA | Zhang, Huiyan,Zhao, Yong,Cao, Hailong,Mou, Guangqing,&Yin, Heng.(2015).Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis.international journal of biological macromolecules,79,72-75. |
| MLA | Zhang, Huiyan,et al."Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis".international journal of biological macromolecules 79(2015):72-75. |
入库方式： OAI收割
来源：大连化学物理研究所
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