The preservation of islet with alginate encapsulation in the process of transportation
文献类型：期刊论文
| 作者 | Li, Na1,2,3; Zhang, Ying2; Xiu, Zhilong1; Wang, Yu2,3; Chen, Li2,3; Wang, Shujun1,2; Li, Shen1,2; Guo, Xin2; Ma, Xiaojun2 |
| 刊名 | biotechnology and applied biochemistry
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 62期号:4页码:530-536 |
| 关键词 | Box-Behnken design cell encapsulation islet survival rate islet transportation response surface methodology |
| 英文摘要 | restoration of insulin secretion by transplantation of isolated islets is a treatment for type diabetes mellitus. one of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. we developed a method that uses alginate encapsulation to protect islets from mechanical damage during shipment. we tested several variables for their impact on islet viability during transportation and used the significant variable to build a response surface methodology (rsm) model by the box-behnken design method. this type of model is a mathematical and statistical technique that we used to optimize the conditions for islet viability during shipment. in this study, the factors that significantly affected islet survival rate were incubation time, serum concentration, and preservation time. then, an empirical model was built to optimize conditions of the islets for shipping according to the responses of the effect factors with rsm. this model can be used to predict the islet survival rate and can serve as a guide for optimizing the transportation method of islets and increasing the success rate of the transplant procedure. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | rat islets ; cryopreservation ; transplantation ; survival ; cells |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360229200013 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146498]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Zhang, Ying,Xiu, Zhilong,et al. The preservation of islet with alginate encapsulation in the process of transportation[J]. biotechnology and applied biochemistry,2015,62(4):530-536. |
| APA | Li, Na.,Zhang, Ying.,Xiu, Zhilong.,Wang, Yu.,Chen, Li.,...&Ma, Xiaojun.(2015).The preservation of islet with alginate encapsulation in the process of transportation.biotechnology and applied biochemistry,62(4),530-536. |
| MLA | Li, Na,et al."The preservation of islet with alginate encapsulation in the process of transportation".biotechnology and applied biochemistry 62.4(2015):530-536. |
入库方式： OAI收割
来源：大连化学物理研究所
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