Sequential enrichment of singly- and multiply-phosphorylated peptides with zwitterionic hydrophilic interaction chromatography material
文献类型：期刊论文
| 作者 | Sheng, Qianying1; Yang, Kaiya1; Xue, Xingya3; Li, Xiuling3,4; Guo, Zhimou3; Shen, Aijin3; Ke, Yanxiong5; Lan, Minbo1,2; Liang, Xinmiao |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2015-09-25 |
| 卷号 | 1413页码:47-59 |
| 关键词 | Zwitterionic material Mixed-mode retention mechanisms Sequential enrichment Multiply-phosphorylated peptides Mass spectrometry |
| 英文摘要 | an interesting and novel method for the selective and sequential enrichment of singly- and multiply-phosphorylated peptides with a zwitterionic material "click te-cys" is presented. retention mechanisms between phosphopeptides and click te-cys are systematically investigated by checking the influence of acetonitrile content, ph value, and buffer concentration on the retention of phosphopeptides. both hydrophilic interaction and electrostatic interaction are involved in retention between phosphopeptides and click te-cys. based on these results, an optimized method is established for selective enrichment of phosphopeptides using click te-cys. this method not only exhibits high selectivity for phosphopeptides, but also fractionates singly- and multiply-phosphorylated peptides into two fractions. this method was evaluated using relatively complex samples, including peptide mixtures of alpha-casein and bovine serum albumin (bsa) at a molar ratio of 1:10 and skim milk. this efficient and optimized protocol has great potential for enriching multiply-phosphorylated peptides and could be a valuable tool for specific enrichment of phosphopeptides in phosphoproteome analysis. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | solid-phase extraction ; interaction liquid-chromatography ; phosphoproteome analysis ; mass-spectrometry ; exchange chromatography ; ion-exchange ; in-vivo ; separation ; phosphopeptides ; combination |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361577600005 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/146558]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 4.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China 5.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sheng, Qianying,Yang, Kaiya,Xue, Xingya,et al. Sequential enrichment of singly- and multiply-phosphorylated peptides with zwitterionic hydrophilic interaction chromatography material[J]. journal of chromatography a,2015,1413:47-59. |
| APA | Sheng, Qianying.,Yang, Kaiya.,Xue, Xingya.,Li, Xiuling.,Guo, Zhimou.,...&Liang, Xinmiao.(2015).Sequential enrichment of singly- and multiply-phosphorylated peptides with zwitterionic hydrophilic interaction chromatography material.journal of chromatography a,1413,47-59. |
| MLA | Sheng, Qianying,et al."Sequential enrichment of singly- and multiply-phosphorylated peptides with zwitterionic hydrophilic interaction chromatography material".journal of chromatography a 1413(2015):47-59. |
入库方式： OAI收割
来源：大连化学物理研究所
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