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New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16

文献类型：期刊论文

作者Ni, Xiangmin1,2; Ru, Heng2; Ma, Feng3; Zhao, Lixia2; Shaw, Neil2; Feng, Yingang4,5; Ding, Wei2; Gong, Weibin2; Wang, Qiaofeng1; Ouyang, Songying2
刊名JOURNAL OF MOLECULAR CELL BIOLOGY
出版日期2016-02-01
卷号8期号:1页码:51-61
关键词interferon gamma-inducible protein 16 (IFI16) hematopoietic interferon-inducible nuclear (HIN) domain DNA recognition innate immune responses
英文摘要Interferon gamma-inducible protein 16 (IFI16) senses DNA in the cytoplasm and the nucleus by using two tandem hematopoietic interferon-inducible nuclear (HIN) domains, HINa and HINb, through the cooperative assembly of IFI16 filaments on double-stranded DNA (dsDNA). The role of HINa in sensing DNA is not clearly understood. Here, we describe the crystal structure of the HINa domain in complex with DNA at 2.55 resolution and provide the first insight into the mode of DNA binding by the HINa domain. The structure reveals the presence of two oligosaccharide/nucleotide-binding (OB) folds with a unique DNA-binding surface. HINa uses loop L-45 of the canonical OB2 fold to bind to the DNA backbone. The dsDNA is recognized as two single strands of DNA. Interestingly, deletion of HINb compromises the ability of IFI16 to induce IFN-beta, while HINa mutants impaired in DNA binding enhance the production of IFN-beta. These results shed light on the roles of IFI16 HIN domains in DNA recognition and innate immune responses.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CYCLIC GMP-AMP ; INNATE IMMUNE-RESPONSE ; TOLL-LIKE RECEPTORS ; DENDRITIC CELLS ; INTRACELLULAR DNA ; CYTOSOLIC DNA ; PROVIDES INSIGHTS ; CYTOPLASMIC DNA ; I INTERFERON ; PROTEIN
收录类别SCI
语种英语
WOS记录号WOS:000372615600006
公开日期2016-05-17
源URL[http://ir.qibebt.ac.cn/handle/337004/8065]  
专题青岛生物能源与过程研究所_代谢物组学团队
作者单位1.Kunming Med Univ, Inst Mol & Clin Med, Kunming 650500, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
6.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China
推荐引用方式
GB/T 7714		 Ni, Xiangmin,Ru, Heng,Ma, Feng,et al. New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2016,8(1):51-61.
APA Ni, Xiangmin.,Ru, Heng.,Ma, Feng.,Zhao, Lixia.,Shaw, Neil.,...&Liu, Zhi-Jie.(2016).New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16.JOURNAL OF MOLECULAR CELL BIOLOGY,8(1),51-61.
MLA Ni, Xiangmin,et al."New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16".JOURNAL OF MOLECULAR CELL BIOLOGY 8.1(2016):51-61.

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来源：青岛生物能源与过程研究所

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