Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe
文献类型：期刊论文
| 作者 | Ji, Wei ; Du, Lingyun ; Zhang, Yuanfu ; Liu, Guofu ; Wang, Shuhao |
| 刊名 | FOOD ANALYTICAL METHODS
 |
| 出版日期 | 2015 |
| 卷号 | 8期号:10页码:2596-2604 |
| 关键词 | LINKED-IMMUNOSORBENT-ASSAY TANDEM MASS-SPECTROMETRY HUMAN EXPOSURE MIGRATION FOOD BPA SAMPLES DNA TEMPERATURE BIOSENSOR |
| ISSN号 | 1936-9751 |
| 中文摘要 | An ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk. |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s12161-015-0162-2 |
| 语种 | 英语 |
| 公开日期 | 2016-05-12 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/9803]  |
| 专题 | 西双版纳热带植物园_文献共享 |
| 推荐引用方式 GB/T 7714 | Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe[J]. FOOD ANALYTICAL METHODS,2015,8(10):2596-2604. |
| APA | Ji, Wei,Du, Lingyun,Zhang, Yuanfu,Liu, Guofu,&Wang, Shuhao.(2015).Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe.FOOD ANALYTICAL METHODS,8(10),2596-2604. |
| MLA | Ji, Wei,et al."Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe".FOOD ANALYTICAL METHODS 8.10(2015):2596-2604. |
入库方式： OAI收割
来源：西双版纳热带植物园
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