Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer
文献类型：期刊论文
| 作者 | Du, Lingyun ; Zhang, Chunyan ; Wang, Longjun ; Liu, Guofu ; Zhang, Yuanfu ; Wang, Shuhao |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2015 |
| 卷号 | 182期号:42433页码:539-545 |
| 关键词 | TANDEM MASS-SPECTROMETRY LIQUID-LIQUID MICROEXTRACTION EXPOSURE RISK EXTRACTION EXPRESSION PROTEINS QUECHERS CANCER SIGNAL |
| ISSN号 | 0026-3672 |
| 中文摘要 | We demonstrate an ultrasensitive time-resolved fluorescence immunoassay for bisphenol A (BPA) where gold nanoparticles (Au-NPs) were modified with anti-BPA antibody and thiolated dsDNA-biotin acting as a signal amplifier. In a competitive reaction, the analyte (BPA) competes with immobilized BPA-ovalbumin conjugate on the surface of microtiter plates to bind to the anti-BPA antibodies on the surface of the Au-NPs. In the next step, a Eu(III)-labeled streptavidin is added to link to the SH-dsDNA-biotin as a tracer. Fluorescence is amplified via both the Au-NPs and the biotin-streptavidin systems, and its intensity is measured in a time-resolved fluorescence immunoassay. The linear range is from 1.0 fga (TM) mL(-1) to 1.0 nga (TM) mL(-1), and the detection limit is 0.3 fg mL(-1), respectively. The method was applied to the determination of BPA in spiked water sample, with recoveries ranging from 90.2 to 106.4 %. |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00604-014-1356-9 |
| 语种 | 英语 |
| 公开日期 | 2016-05-12 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/9812]  |
| 专题 | 西双版纳热带植物园_文献共享 |
| 推荐引用方式 GB/T 7714 | Du, Lingyun,Zhang, Chunyan,Wang, Longjun,et al. Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer[J]. MICROCHIMICA ACTA,2015,182(42433):539-545. |
| APA | Du, Lingyun,Zhang, Chunyan,Wang, Longjun,Liu, Guofu,Zhang, Yuanfu,&Wang, Shuhao.(2015).Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer.MICROCHIMICA ACTA,182(42433),539-545. |
| MLA | Du, Lingyun,et al."Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer".MICROCHIMICA ACTA 182.42433(2015):539-545. |
入库方式： OAI收割
来源：西双版纳热带植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。