Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S
文献类型：期刊论文
| 作者 | Liu, YH; Hu, XK ; Liu, H
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| 刊名 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 107页码:56-61 |
| 关键词 | Acinetobacter sp. Industrial-scale fermentation Long-term storage Crude oil-degrading bacterium |
| ISSN号 | 0964-8305 |
| 产权排序 | [Liu, Yuhua; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Beijing 100864, Peoples R China; [Liu, Yuhua] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Hui] Diyuan Biol Technol Co Ltd, Yantai, Peoples R China |
| 通讯作者 | Hu, XK (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Beijing 100864, Peoples R China. xkhu@yic.ac.cn |
| 英文摘要 | The marine bacterium Acinetobacter sp. strain HC8-3S, capable of degrading petroleum hydrocarbons, was previously shown to be applicable for bioremediation. Here, we evaluated the performance of the strain during industrial-scale fermentation, long-term storage, and biodegradation processes. Fermentation parameters were optimized for industrial-scale culturing using lower salinity and fewer inorganic salts in the culture medium. The shelf-life of the fermentation broth was evaluated in different storage conditions. Biodegradation efficiency of the strain was evaluated using gas chromatography. Results indicated that the optimum conditions for one-ton scale fermentation were 30 degrees C, pH 7.4-7.8, and rotation speed 90 rpm; the cell density reached 3.6 +/- 1.9 x 10(10) CFU ml(-1) after 12 h. The low temperature can preserved fermentation broth for longer time. When the storage temperature was down to 4 degrees C from room temperature, half-life of strain HC8-3S extended from 9 days to 34 days. The biodegradation rate of the saturated hydrocarbon fraction of crude oil was 94% after treatment with the strain for 5 days. The results indicate that Acinetobacter sp. strain HC8-3S can be enriched efficiently on a large scale, making it a potentially useful industrial strain. This crude oil degradative capability of the strain HC8-3S provides possible application for the clean-up of crude oil-contaminated environment. (C) 2015 Elsevier Ltd. All rights reserved. |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| 关键词[WOS] | BACTERIAL COMMUNITY RESPONSE ; PETROLEUM-HYDROCARBONS ; FRESH-WATER ; BIODEGRADATION ; BIOREMEDIATION ; SEA ; DEGRADATION ; POLLUTANTS ; SEDIMENT ; GULF |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369206700008 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/10878]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat 2.Univ Chinese Acad Sci 3.Diyuan Biol Technol Co Ltd |
| 推荐引用方式 GB/T 7714 | Liu, YH,Hu, XK,Liu, H. Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2016,107:56-61. |
| APA | Liu, YH,Hu, XK,&Liu, H.(2016).Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,107,56-61. |
| MLA | Liu, YH,et al."Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 107(2016):56-61. |
入库方式： OAI收割
来源：烟台海岸带研究所
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