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A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA
文献类型：期刊论文
| 作者 | Fu, X; Cheng, Z; Yu, J; Choo, P; Chen, L; Choo, J |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2016-04-15 |
| 卷号 | 78页码:530-537 |
| 关键词 | Human immunodeficiency virus type 1 (HIV-1) Surface-enhanced Raman scattering Gold nanoparticles DNA Lateral flow assay |
| ISSN号 | 0956-5663 |
| 产权排序 | [Fu, Xiuli; Cheng, Ziyi; Yu, Jimin; Choo, Jaebum] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea; [Choo, Priscilla] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 通讯作者 | Choo, J (reprint author), Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea. jbchoo@hanyang.ac.kr |
| 英文摘要 | User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity. (C) 2015 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | SURFACE-ENHANCED RAMAN ; FUNCTIONALIZED GOLD NANOPARTICLES ; DETECTION PLATFORM ; VISUAL DETECTION ; STRIP BIOSENSOR ; IMMUNOASSAY ; SCATTERING ; ELISA ; SPECTROSCOPY ; SILVER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000368954900074 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/10874]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Hanyang Univ, Dept Bionano Technol 2.Indiana Univ, Dept Chem 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat |
| 推荐引用方式 GB/T 7714 | Fu, X,Cheng, Z,Yu, J,et al. A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA[J]. BIOSENSORS & BIOELECTRONICS,2016,78:530-537. |
| APA | Fu, X,Cheng, Z,Yu, J,Choo, P,Chen, L,&Choo, J.(2016).A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA.BIOSENSORS & BIOELECTRONICS,78,530-537. |
| MLA | Fu, X,et al."A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA".BIOSENSORS & BIOELECTRONICS 78(2016):530-537. |
入库方式： OAI收割
来源：烟台海岸带研究所
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