A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium
文献类型：期刊论文
| 作者 | Xiao, LL ; Sun, QBA; Lian, B
|
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 72期号:4页码:410-419 |
| ISSN号 | 0343-8651 |
| 产权排序 | [Xiao, Leilei; Sun, Qibiao; Lian, Bin] Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China; [Xiao, Leilei] Chinese Acad Sci, Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China |
| 通讯作者 | Lian, B (reprint author), Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China. ai-yanzi@163.com ; sunqibiao001@163.com ; bin2368@vip.163.com |
| 英文摘要 | Many researchers have suggested that microbes can accelerate the weathering of silicate minerals. However, many genes and metabolic pathways related to microorganisms obtaining potassium (K) from silicate remain undiscovered. It is feasible to detect the gene expression within the scope of the whole genome through high-throughput sequencing. Surprisingly, only a few reports have shown fungal weathering of silicate using this technology. This study explored differences in gene expression of Aspergillus nidulans, which was cultured with different K sources, KCl, and K-feldspar. A. nidulans RNA was extracted by the construction of a cDNA library. Identification of K-acquisition-related genes with GO and KEGG pathway analysis revealed that primarily differentially expressed genes were related to the biosynthesis of amino acids. When these genes were grouped in accordance with the physiological functions, the genes involved in the synthesis of protease, ribosome, and mitochondria, trans-membrane transport, and oxidative phosphorylation were significantly different. Moreover, 20 genes selected were further tested using RT-qPCR. One half (10 genes) exhibited differential expression, which was consistent with the results of RNA-seq. Combining the results of RNA-seq and RT-qPCR, we summarised a possible way to obtain mineral K in A. nidulans as well. The differentially expressed genes and their associated metabolic pathways revealed will improve the understanding of the molecular mechanisms of microbial weathering of silicate minerals. |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | CARBONIC-ANHYDRASE ; BEARING MINERALS ; ARABIDOPSIS ; STRESS ; ECTOMYCORRHIZAL ; TRANSCRIPTOME ; SYSTEM ; EUKARYOTES ; PATHWAYS ; BIOTITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000371250500008 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/10841]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom 2.Chinese Acad Sci, Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat |
| 推荐引用方式 GB/T 7714 | Xiao, LL,Sun, QBA,Lian, B. A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium[J]. CURRENT MICROBIOLOGY,2016,72(4):410-419. |
| APA | Xiao, LL,Sun, QBA,&Lian, B.(2016).A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium.CURRENT MICROBIOLOGY,72(4),410-419. |
| MLA | Xiao, LL,et al."A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium".CURRENT MICROBIOLOGY 72.4(2016):410-419. |
入库方式： OAI收割
来源：烟台海岸带研究所
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