Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe
文献类型：期刊论文
| 作者 | Hu,Zhenzhen; Chen,Jian; Li,Wenying; Wang,Yan; Li,Yongxin; Sang,Lijia; Li,Juanmin; Zhang,Qingfeng; Ibupoto,Zafar Hussain; Yu,Cong |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2015 |
| 卷号 | 895期号:37页码:89-94 |
| 关键词 | SIGNAL AMPLIFICATION HUMAN CANCERS QUANTIFICATION EXPRESSION HYBRIDIZATION STRATEGY POLYMER ASSAY MIRNA |
| 通讯作者 | yu,c |
| 英文摘要 | a novel fluorescence turn-on microrna (mirna) detection method based on duplex-specific nuclease (dsn) and a perylene probe is presented in this study. a positively charged perylene derivative (compound 1) was used as the fluorescent probe. compound 1 exhibits strong monomer fluorescence in an aqueous buffer solution. it is well known that single-stranded dna is a polyanion in nature. thus, it can induce the aggregation of compound 1 through strong electrostatic, hydrophobic and pi-pi stacking interactions. as a result, the fluorescence of compound 1 was efficiently quenched. when the target mirna was added, the formation of dna-rna hybridized duplex initiated the cleavage of the dna strand by dsn cycle reaction, which resulted in disaggregation of compound 1. a fluorescence turn-on signal was detected, and a novel mirna sensing method was therefore established. the presented method is label-free, simple, cost effective, sensitive and selective. (c) 2015 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000362945600009 |
| 公开日期 | 2016-05-31 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/65124]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu,Zhenzhen,Chen,Jian,Li,Wenying,et al. Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe[J]. analytica chimica acta,2015,895(37):89-94. |
| APA | Hu,Zhenzhen.,Chen,Jian.,Li,Wenying.,Wang,Yan.,Li,Yongxin.,...&Yu,Cong.(2015).Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe.analytica chimica acta,895(37),89-94. |
| MLA | Hu,Zhenzhen,et al."Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe".analytica chimica acta 895.37(2015):89-94. |
入库方式： OAI收割
来源：长春应用化学研究所
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