A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles
文献类型：期刊论文
| 作者 | Hu,Jingting; Ni,Pengjuan; Dai,Haichao; Sun,Yujing; Wang,Yilin; Jiang,Shu; Li,Zhuang |
| 刊名 | rsc advances
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:21页码:16036-16041 |
| 关键词 | POLYMERASE-CHAIN-REACTION IRON-OXIDE NANOPARTICLES IMMUNOASSAY BIOSENSOR SELECTION PROTEINS GLUCOSE TOXIN ASSAY ENRICHMENT |
| 通讯作者 | li,z |
| 英文摘要 | aunps possess peroxidase-like activity that could catalyze 3,3,5,5-tetramethylbenzidine (tmb) in the presence of h2o2, leading to color change of the solution. herein we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of ricin by using aunps. it is shown that the peroxidase-like activity of aunps can be improved by surface activation with target-specific aptamers. however, with target molecules, the aptamer is desorbed from the aunps' surface, resulting in a decrease of the catalytic abilities of aunps. the color change of the solution was relevant to the target concentration, and this can be judged by the naked eye and monitored by uv-vis spectrometry. the linear range for the current analytical system was from 0.05 nm to 10 nm. the corresponding limit of detection (lod) was 0.05 nm. some other proteins such as thrombin (th), glucose oxidase (gox), and bovine albumin (bsa) all had a negligible effect on the determination of ricin. what is more, several practical samples spiked with ricin were analyzed using the proposed method with excellent recoveries. this colorimetric aptasensor is superior to the other conventional methods owing to its simplicity, low cost, and high sensitivity. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-31 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/67820]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu,Jingting,Ni,Pengjuan,Dai,Haichao,et al. A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles[J]. rsc advances,2015,5(21):16036-16041. |
| APA | Hu,Jingting.,Ni,Pengjuan.,Dai,Haichao.,Sun,Yujing.,Wang,Yilin.,...&Li,Zhuang.(2015).A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles.rsc advances,5(21),16036-16041. |
| MLA | Hu,Jingting,et al."A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles".rsc advances 5.21(2015):16036-16041. |
入库方式： OAI收割
来源：长春应用化学研究所
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