Spheres-on-sphere silica microspheres as matrix for horseradish peroxidase immobilization and detection of hydrogen peroxide
文献类型：期刊论文
| 作者 | Lei,Zhen; Liu,Xia; Ma,Lina; Liu,Dianjun; Zhang,Haifei; Wang,Zhenxin |
| 刊名 | rsc advances
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:48页码:38665-38672 |
| 关键词 | NEURODEGENERATIVE DISEASES NANOCRYSTALLINE DIAMOND ENZYME IMMOBILIZATION CATALYTIC-ACTIVITY OXIDATIVE STRESS STABILITY SUPPORT NANOPARTICLES BIOSENSOR GLUCOSE |
| 通讯作者 | wang,zx |
| 英文摘要 | in this work, spheres-on-sphere (sos) silica microspheres are prepared via a facile one-pot synthesis. after functionalization with carboxyl groups, the carboxylated sos silica microspheres (sos-cooh microspheres) can serve as a support for horseradish peroxidase (hrp) covalent immobilization. the obtained enzyme hybrid (sos-cooh-hrp) is more stable under alkaline conditions than the free counterpart, and exhibits longer-term storage stability and higher resistance toward the denaturing agents such as guanidine hydrochloride (gdmcl) and urea. the michaelis-menten constant (k-m) of the immobilized enzyme is decreased slightly while the maximum rate of reaction (v-max) is very close to that of free hrp, resulting in the catalytic efficiency of sos-cooh-hrp being enhanced significantly. for evaluating its utility, a sos-cooh-hrp-based colorimetric method has been developed for selectively and sensitively detecting h2o2 both in buffer and 10% diluted human serum. furthermore, the sos-cooh-hrp displays excellent reusability and reproducibility in cycle analysis. the results demonstrate that the sos-cooh-hrp has great potential for practical applications in biosensing and industrial fields. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-31 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/67843]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lei,Zhen,Liu,Xia,Ma,Lina,et al. Spheres-on-sphere silica microspheres as matrix for horseradish peroxidase immobilization and detection of hydrogen peroxide[J]. rsc advances,2015,5(48):38665-38672. |
| APA | Lei,Zhen,Liu,Xia,Ma,Lina,Liu,Dianjun,Zhang,Haifei,&Wang,Zhenxin.(2015).Spheres-on-sphere silica microspheres as matrix for horseradish peroxidase immobilization and detection of hydrogen peroxide.rsc advances,5(48),38665-38672. |
| MLA | Lei,Zhen,et al."Spheres-on-sphere silica microspheres as matrix for horseradish peroxidase immobilization and detection of hydrogen peroxide".rsc advances 5.48(2015):38665-38672. |
入库方式： OAI收割
来源：长春应用化学研究所
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