Investigating interactions of the Bacillus subtilis spore coat proteins CotY and CotZ using single molecule force spectroscopy
文献类型：期刊论文
| 作者 | Liu,Huiqing; Krajcikova,Daniela; Zhang,Zhe; Wang,Hongda; Barak,Imrich; Tang,Jilin |
| 刊名 | journal of structural biology
 |
| 出版日期 | 2015 |
| 卷号 | 192期号:1页码:14-20 |
| 关键词 | SPORULATION LAYER LOCALIZATION GERMINATION MICROSCOPE DYNAMICS NETWORK COMPLEX CELLS |
| 通讯作者 | tang,jl |
| 英文摘要 | spores formed by bacillus subtilis are surrounded by a protective and multilayered shell, termed the coat, which grants the spores resistance to various environmental stresses and facilitates spore germination. the spore coat consists of more than seventy different proteins, arranged into at least four distinct structural layers: the undercoat, inner coat, outer coat and crust. however, how these proteins, especially the morphogenetic proteins, interact to establish the organized, functional coat layers remains poorly understood. coty and cotz as the components of the crust, play a morphogenetic role in the crust assembly around the spore. in this study, the single molecule force spectroscopy was used to investigate the interaction and dynamics between coty and cotz at the single-molecule level. the results show that homotypic interactions of coty and cotz and the heterotypic interaction between coty and cotz exist. furthermore, the dissociation kinetics of the complexes were studied by monitoring the relationship between the unbinding forces and the loading rates at different pulling velocities. in this way, a series of kinetic parameters regarding the three different complexes were obtained. it revealed the strong interactions between coty and cotz, coty and coty, and a relatively weak interaction of cotz and cotz. (c) 2015 elsevier inc. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-31 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/67926]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu,Huiqing,Krajcikova,Daniela,Zhang,Zhe,et al. Investigating interactions of the Bacillus subtilis spore coat proteins CotY and CotZ using single molecule force spectroscopy[J]. journal of structural biology,2015,192(1):14-20. |
| APA | Liu,Huiqing,Krajcikova,Daniela,Zhang,Zhe,Wang,Hongda,Barak,Imrich,&Tang,Jilin.(2015).Investigating interactions of the Bacillus subtilis spore coat proteins CotY and CotZ using single molecule force spectroscopy.journal of structural biology,192(1),14-20. |
| MLA | Liu,Huiqing,et al."Investigating interactions of the Bacillus subtilis spore coat proteins CotY and CotZ using single molecule force spectroscopy".journal of structural biology 192.1(2015):14-20. |
入库方式： OAI收割
来源：长春应用化学研究所
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