Vertically Scanned Laser Sheet Microscopy
文献类型：期刊论文
| 作者 | Di Dong1; Alicia Arranz2; ShouPing Zhu3; Yujie Yang1; Liangliang Shi1; Jun Wang4; Chen Shen3; Jie Tian1; Jorge Ripoll5,6,7; Dong, Di
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| 刊名 | Journal of Biomedical Optics
 |
| 出版日期 | 2014-10 |
| 卷号 | 19期号:10页码:106001-8 |
| 关键词 | light sheet microscopy selective plane illumination microscopy stripe artifact |
| 通讯作者 | Jie Tian, Jorge Ripoll |
| 英文摘要 | Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345837200018 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/11158]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.aChinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China 2.Swiss Federal Institute of Technology (ETH-Zurich), Institute for Biomedical Engineering, Switzerland 3.Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China 4.Harbin University of Science and Technology, School of Automation, Harbin 150080, China 5.Universidad Carlos III of Madrid, Department of Bioengineering and Aerospace Engineering, Madrid 28911, Spain 6.Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, Experimental Medicine and Surgery Unit, Madrid 28007, Spain 7.Foundation for Research and Technology-Hellas, Institute of Electronic Structure and Laser, Heraklion 71110, Greece |
| 推荐引用方式 GB/T 7714 | Di Dong,Alicia Arranz,ShouPing Zhu,et al. Vertically Scanned Laser Sheet Microscopy[J]. Journal of Biomedical Optics,2014,19(10):106001-8. |
| APA | Di Dong.,Alicia Arranz.,ShouPing Zhu.,Yujie Yang.,Liangliang Shi.,...&Wang, Jun.(2014).Vertically Scanned Laser Sheet Microscopy.Journal of Biomedical Optics,19(10),106001-8. |
| MLA | Di Dong,et al."Vertically Scanned Laser Sheet Microscopy".Journal of Biomedical Optics 19.10(2014):106001-8. |
入库方式： OAI收割
来源：自动化研究所
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